Grupo de Fisiología del Estrés en Plantas (Dpto. de Biología Ambiental), Unidad Asociada al CSIC, EEAD, Zaragoza e ICVV, Logroño, Facultades de Ciencias y Farmacia, Universidad de Navarra, Irunlarrea 1, 31008 Pamplona, Spain; INRA, Institut des Sciences de la Vigne et du Vin, UMR1287 EGFV, 210 Chemin de Leysotte, 33883 Villenave D'Ornon, France; University of Bordeaux, Institut des Sciences de la Vigne et du Vin, UMR1287 EGFV, 210 Chemin de Leysotte, 33883 Villenave D'Ornon, France.
Estación Experimental de Aula Dei (EEAD), CSIC, Dpto. Nutrición Vegetal, Apdo. 13034, 50080 Zaragoza, Spain; Grupo de Fisiología del Estrés en Plantas (Dpto. de Biología Ambiental), Unidad Asociada al CSIC, EEAD, Zaragoza e ICVV, Logroño, Facultades de Ciencias y Farmacia, Universidad de Navarra, Irunlarrea 1, 31008 Pamplona, Spain.
Plant Sci. 2015 Mar;232:13-22. doi: 10.1016/j.plantsci.2014.12.013. Epub 2014 Dec 23.
This work aims to characterize the physiological response of grapevine (Vitis vinifera L.) cv. Tempranillo to UV-B radiation under water deficit conditions. Grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective UV-B radiation (0, 5.98 and 9.66kJm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. UV-B induced a transient decrease in net photosynthesis (Anet), actual and maximum potential efficiency of photosystem II, particularly on well watered plants. Methanol extractable UV-B absorbing compounds (MEUVAC) concentration and superoxide dismutase activity increased with UV-B. Water deficit effected decrease in Anet and stomatal conductance, and did not change non-photochemical quenching and the de-epoxidation state of xanthophylls, dark respiration and photorespiration being alternative ways to dissipate the excess of energy. Little interactive effects between UV-B and drought were detected on photosynthesis performance, where the impact of UV-B was overshadowed by the effects of water deficit. Grape berry ripening was strongly delayed when UV-B and water deficit were applied in combination. In summary, deficit irrigation did not modify the adaptive response of grapevine to UV-B, through the accumulation of MEUVAC. However, combined treatments caused additive effects on berry ripening.
本研究旨在探究水分亏缺条件下，UV-B 辐射对葡萄（Vitis vinifera L.）品种 Tempranillo 生理响应的影响。在温室控制条件下，采用完全随机区组设计，以果实着生至成熟为时间进程，对结果枝进行 3 个水平（0、5.98 和 9.66kJm(-2)day(-1))的补充生物有效 UV-B 辐射和 2 个水分处理（充分供水和水分亏缺）。UV-B 导致净光合速率（Anet）、实际和最大光系统 II 潜在效率的短暂下降，在充分供水的植株中表现尤为明显。甲醇可提取的 UV-B 吸收化合物（MEUVAC）浓度和超氧化物歧化酶活性随 UV-B 增加。水分亏缺导致 Anet 和气孔导度下降，但不改变非光化学猝灭和叶黄素脱环氧化状态、暗呼吸和光呼吸成为耗散过剩能量的替代途径。在光合作用性能方面，UV-B 和干旱之间的互作效应很小，UV-B 的影响被水分亏缺的影响所掩盖。当 UV-B 和水分亏缺联合应用时，葡萄浆果的成熟会严重延迟。总之，亏缺灌溉并没有通过 MEUVAC 的积累来改变葡萄对 UV-B 的适应反应。然而，联合处理对浆果成熟有累加效应。
