Silva S, Sepodes B, Rocha J, Direito R, Fernandes A, Brites D, Freitas M, Fernandes E, Bronze M R, Figueira M E
Ibet, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, 2780-157 Oeiras, Portugal; Itqb, Estação Agronómica Nacional, Av. da República, 2780-157 Oeiras, Portugal; Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal; iMED.ULisboa, Research Institute for Medicines, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
J Nutr Biochem. 2015 Apr;26(4):360-8. doi: 10.1016/j.jnutbio.2014.11.011. Epub 2014 Dec 30.
Virgin olive oil is the primary source of fat in the Mediterranean diet, and its beneficial health effects have been related with oleic acid and phenolic compounds content. Hydroxytyrosol, a typical virgin olive oil phenolic compound, has beneficial antioxidant and anti-inflammatory properties as previously reported. The aim of this study was to evaluate the effect of hydroxytyrosol-supplemented refined olive oil at 0.5 and 5 mg/kg in a rodent model of rheumatoid arthritis. Rheumatoid arthritis was induced by intradermic administration, in male Wistar rats, of Freund's adjuvant with collagen type II on days 1 and 21. Hydroxytyrosol-supplemented refined olive oils were administrated by gavage from day 23 until day 35. The treatment at 5-mg/kg dose significantly decreased paw edema (P<.01), histological damage, cyclooxygenase-2 and inducible nitric oxide synthase expression, and markedly reduced the degree of bone resorption, soft tissue swelling and osteophyte formation, improving articular function in treated animals. Acute inflammation, induced by carrageenan, was also evaluated for hydroxytyrosol-supplemented refined olive oils at 0.5 and 5 mg/kg. Both doses significantly reduced paw edema (P<.001). Our results suggest that the supplementation of refined olive oil with hydroxytyrosol may be advantageous in rheumatoid arthritis with significant impact not only on chronic inflammation but also on acute inflammatory processes.
初榨橄榄油是地中海饮食中脂肪的主要来源，其有益健康的作用与油酸和酚类化合物的含量有关。正如先前报道的那样，羟基酪醇是初榨橄榄油中的一种典型酚类化合物，具有有益的抗氧化和抗炎特性。本研究的目的是评估在类风湿性关节炎啮齿动物模型中，补充0.5毫克/千克和5毫克/千克羟基酪醇的精制橄榄油的效果。在雄性Wistar大鼠的第1天和第21天，通过皮内注射弗氏佐剂和II型胶原蛋白诱导类风湿性关节炎。从第23天到第35天，通过灌胃给予补充羟基酪醇的精制橄榄油。5毫克/千克剂量的治疗显著减轻了爪部水肿（P<0.01）、组织学损伤、环氧合酶-2和诱导型一氧化氮合酶的表达，并显著降低了骨吸收、软组织肿胀和骨赘形成的程度，改善了治疗动物的关节功能。还评估了补充0.5毫克/千克和5毫克/千克羟基酪醇的精制橄榄油对角叉菜胶诱导的急性炎症的作用。两个剂量均显著减轻了爪部水肿（P<0.001）。我们的结果表明，在精制橄榄油中补充羟基酪醇可能对类风湿性关节炎有益，不仅对慢性炎症有显著影响，而且对急性炎症过程也有显著影响。
