Farzannia A, Roghanian R, Zarkesh-Esfahani S H, Nazari M, Emamzadeh R
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.
Analyst. 2015 Mar 7;140(5):1438-41. doi: 10.1039/c4an01946f. Epub 2015 Jan 27.
A novel and advanced Fc-binding probe – FcUni-RLuc namely – has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence – HWRGWV – was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells.
一种新型先进的Fc结合探针——即FcUni-RLuc——已被制备出来并对其标记IgG的功能进行了检测。Fc抗体结合序列——HWRGWV——与海肾荧光素酶融合，纯化后的探针被用于对Her2阳性细胞进行生物发光酶联免疫吸附测定。
