Wang Hao-hua, Zhang Xiu-wei, Chen Wei-kun, Huang Qiu-xia, Chen Qiao-qiong
Department of Endocrinology, Dongguan People's Hospital, Dongguan 523000, Guangdong Province, P. R. China.
Department of Endocrinology, Dongguan People's Hospital, Dongguan 523000, Guangdong Province, P. R. China.
J Diabetes Complications. 2015 Apr;29(3):384-9. doi: 10.1016/j.jdiacomp.2015.01.001. Epub 2015 Jan 9.
Irisin is first discovered as a potential mediator of obesity related energy homeostasis. Recent studies indicate that irisin is associated with endothelial dysfunction and atherosclerosis in patients with type 2 diabetes. Our objective was to examine the relationship between irisin and urinary albumin excretion in patients with type 2 diabetes.
100 newly diagnosed patients with type 2 diabetes and 100 healthy subjects were selected. Serum irisin levels were measured by ELISA, and urine albumin was measured by radioimmunoassay. High resolution ultrasound was used to measure brachial artery diameter at rest, after reactive hyperemia (flow-mediated arterial dilation, FMD) and after sublingual glyceryltrinitrate.
Patients with type 2 diabetes presented decreased irisin levels when compared to controls (14.12±3.93 versus 28.98±2.56ng/ml, P=0.015).Serum irisin levels in the microalbuminuric and macroalbuminuria subgroup were 9.89±1.56ng/ml and 5.67±1.89ng/ml, respectively, which were significantly lower than those in the normoalbuminuria (15.97±3.12ng/ml). In comparison to microalbuminuric subgroup, macroalbuminuria subgroup had lower levels of irisin. By dividing the distribution of serum irisin levels into quartiles, FMD was increased gradually with the increase of serum irisin levels (P<0.001). Multiple stepwise linear regression analysis showed that FMD (β=0.75, P=0.002), 2-hBG (β=-0.25, P=0.038) and UAE (β=-0.87, P=0.008) were significantly associated with irisin. Pearson's correlation analyses showed a negative correlation between irisin and logUAE (r=-0.57) and between FMD and logUAE (r=-0.47), and positive correlations between irisin and FMD (r=0.51).
Decreased plasma levels of irisin seem to be associated with UAE and FMD in patients with type 2 diabetes.
鸢尾素最初被发现是肥胖相关能量稳态的潜在调节因子。近期研究表明，鸢尾素与2型糖尿病患者的内皮功能障碍和动脉粥样硬化有关。我们的目的是研究2型糖尿病患者中鸢尾素与尿白蛋白排泄之间的关系。
选取100例新诊断的2型糖尿病患者和100例健康受试者。采用酶联免疫吸附测定法（ELISA）检测血清鸢尾素水平，采用放射免疫分析法检测尿白蛋白。使用高分辨率超声测量静息时、反应性充血后（血流介导的血管舒张，FMD）和舌下含服硝酸甘油后的肱动脉直径。
与对照组相比，2型糖尿病患者的鸢尾素水平降低（14.12±3.93对28.98±2.56ng/ml，P = 0.015）。微量白蛋白尿和大量白蛋白尿亚组的血清鸢尾素水平分别为9.89±1.56ng/ml和5.67±1.89ng/ml，显著低于正常白蛋白尿组（15.97±3.12ng/ml）。与微量白蛋白尿亚组相比，大量白蛋白尿亚组的鸢尾素水平更低。将血清鸢尾素水平分布分为四分位数，随着血清鸢尾素水平升高，FMD逐渐增加（P<0.001）。多元逐步线性回归分析显示，FMD（β = 0.75，P = 0.002）、2小时血糖（β = -0.25，P = 0.038）和尿白蛋白排泄量（UAE，β = -0.87，P = 0.008）与鸢尾素显著相关。Pearson相关分析显示，鸢尾素与logUAE呈负相关（r = -0.57），FMD与logUAE呈负相关（r = -0.47），鸢尾素与FMD呈正相关（r = 0.51）。
2型糖尿病患者血浆鸢尾素水平降低似乎与UAE和FMD有关。
