Peng Chuangang, Zhang Qiao, Yang Qi, Zhu Qingsan
Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130029, Jilin Province, China.
Department of Orthopedics, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
Neural Regen Res. 2012 Aug 15;7(23):1779-85. doi: 10.3969/j.issn.1673-5374.2012.23.003.
A 10-mm long sciatic nerve injury model was established in fresh normal Chinese patient cadavers. Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens. Sciatic nerve injury models were respectively anastomosed using the autologous cadaveric sciatic nerve and human amniotic membrane. Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group. The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity. Experimental findings suggested that human amniotic membranes and autologous sciatic nerves exhibit similar stress-strain curves, good elastic properties, and certain strain and stress capabilities in anastomosis of the injured sciatic nerve.
在新鲜的正常中国患者尸体上建立了10毫米长的坐骨神经损伤模型。从健康产妇的胎盘采集羊膜，并制备成多层、卷曲的管状标本。分别使用自体尸体坐骨神经和人羊膜对坐骨神经损伤模型进行吻合。拉伸试验结果表明，与人羊膜吻合的坐骨神经损伤模型的最大负荷、最大位移、最大应力和最大应变均大于自体神经吻合组。人羊膜和坐骨神经的应力-应变曲线在第一阶段呈指数变化，在第二和第三阶段变为弹性变形曲线，在第四阶段呈现塑性变形曲线，此时标本失去承载能力。实验结果表明，人羊膜和自体坐骨神经在损伤坐骨神经吻合中表现出相似的应力-应变曲线、良好的弹性性能以及一定的应变和应力能力。
