Shokri Rasoul, Salouti Mojtaba, Zanjani Rahim Sorouri
Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Fars, Iran.
J Microbiol. 2015 Feb;53(2):116-21. doi: 10.1007/s12275-015-4519-4. Epub 2015 Jan 28.
The high prevalence of methicillin resistant Staphylococcus aureus (MRSA) and developing resistance to antibiotics requires new approaches for treatment of infectious diseases due to this bacterium. In this study, we developed a targeting agent for selective killing of MRSA using photothermal therapy method based on anti protein A antibody and gold nanorods (GNRs). Polystyrene sulfonate (PSS) coated GNRs were conjugated with anti protein A antibody. The FT-IR and UV-vis analyses approved the formation of anti protein A antibody-gold nanorods conjugate. In vitro study of photothermal therapy showed 82% reduction in the MRSA cells viability which was significantly greater than the ablation effect of free GNRs and laser alone. Significant accumulation of anti protein A antibody-GNRs in the infected muscle in comparison with normal muscle approved the targeting ability of new agent. In vivo study of photothermal therapy resulted in a significant reduction (73%) in the bacterial cells viability in the infected mouse model. These results demonstrated the ability of anti protein A antibody-GNRs conjugate in combination with NIR laser energy for selective killing of MRSA in mouse model.
耐甲氧西林金黄色葡萄球菌（MRSA）的高流行率以及对抗生素产生的耐药性，使得针对这种细菌引起的传染病需要新的治疗方法。在本研究中，我们基于抗蛋白A抗体和金纳米棒（GNRs），利用光热疗法开发了一种用于选择性杀死MRSA的靶向剂。用聚苯乙烯磺酸盐（PSS）包覆的GNRs与抗蛋白A抗体偶联。傅里叶变换红外光谱（FT-IR）和紫外-可见光谱（UV-vis）分析证实了抗蛋白A抗体-金纳米棒偶联物的形成。光热疗法的体外研究表明，MRSA细胞活力降低了82%，这显著大于游离GNRs和单独激光的消融效果。与正常肌肉相比，抗蛋白A抗体-GNRs在感染肌肉中的显著积累证实了新型药剂的靶向能力。光热疗法的体内研究导致感染小鼠模型中的细菌细胞活力显著降低（73%）。这些结果证明了抗蛋白A抗体-GNRs偶联物与近红外（NIR）激光能量相结合在小鼠模型中选择性杀死MRSA的能力。
