Pawar Aditya S, Zhu Xiang-Yang, Eirin Alfonso, Tang Hui, Jordan Kyra L, Woollard John R, Lerman Amir, Lerman Lilach O
The Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, USA.
Obesity (Silver Spring). 2015 Feb;23(2):399-407. doi: 10.1002/oby.20971. Epub 2014 Dec 31.
To establish and characterize a novel domestic porcine model of obesity.
Fourteen domestic pigs were fed normal (lean, n=7) or high-fat/high-fructose diet (obese, n=7) for 16 weeks. Subcutaneous abdominal adipose tissue biopsies were obtained after 8, 12, and 16 weeks of diet, and pericardial adipose tissue after 16 weeks, for assessments of adipocyte size, fibrosis, and inflammation. Adipose tissue volume and cardiac function were studied with multidetector computed tomography, and oxygenation was studied with magnetic resonance imaging. Plasma lipids profile, insulin resistance, and markers of inflammation were evaluated.
Compared with lean pigs, obese pigs had elevated cholesterol and triglyceride levels, blood pressure, and insulin resistance. Both abdominal and pericardial fat volume increased in obese pigs after 16 weeks. In abdominal subcutaneous adipose tissue, adipocyte size and both tumor necrosis factor (TNF)-α expression progressively increased. Macrophage infiltration showed in both abdominal and pericardial adipose tissues. Circulating TNF-α increased in obese pigs only at 16 weeks. Compared with lean pigs, obese pigs had similar global cardiac function, but myocardial perfusion and oxygenation were significantly impaired.
A high-fat/high-fructose diet induces in domestic pigs many characteristics of metabolic syndrome, which is useful for investigating the effects of the obesity.
建立并表征一种新型的国内肥胖猪模型。
14头家猪分别给予正常饮食（瘦猪，n = 7）或高脂/高果糖饮食（肥胖猪，n = 7），持续16周。在饮食8周、12周和16周后获取腹部皮下脂肪组织活检样本，16周后获取心包脂肪组织样本，用于评估脂肪细胞大小、纤维化和炎症。使用多排螺旋计算机断层扫描研究脂肪组织体积和心脏功能，使用磁共振成像研究氧合情况。评估血浆脂质谱、胰岛素抵抗和炎症标志物。
与瘦猪相比，肥胖猪的胆固醇、甘油三酯水平、血压和胰岛素抵抗均升高。16周后，肥胖猪的腹部和心包脂肪体积均增加。在腹部皮下脂肪组织中，脂肪细胞大小和肿瘤坏死因子（TNF）-α表达均逐渐增加。腹部和心包脂肪组织均出现巨噬细胞浸润。肥胖猪仅在16周时循环TNF-α升高。与瘦猪相比，肥胖猪的整体心脏功能相似，但心肌灌注和氧合明显受损。
高脂/高果糖饮食可诱导家猪出现许多代谢综合征特征，这对于研究肥胖的影响很有用。
