Vieira-Potter Victoria J, Lee Sewon, Bayless David S, Scroggins Rebecca J, Welly Rebecca J, Fleming Nicholas J, Smith Thomas N, Meers Grace M, Hill Michael A, Rector R Scott, Padilla Jaume
Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri, USA.
Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri, USA.
Obesity (Silver Spring). 2015 Dec;23(12):2421-9. doi: 10.1002/oby.21252. Epub 2015 Nov 2.
The Ossabaw pig is emerging as an attractive model of human cardiometabolic disease because of its size and susceptibility to atherosclerosis, among other characteristics. The relationship between adipose tissue inflammation and metabolic dysfunction in this model was investigated here.
Young female Ossabaw pigs were fed a Western-style high-fat diet (HFD) (n = 4) or control low-fat diet (LFD) (n = 4) for a period of 9 months and compared for cardiometabolic outcomes and adipose tissue inflammation.
The HFD-fed "OBESE" pigs were 2.5 times heavier (P < 0.001) than LFD-fed "LEAN" pigs and developed severe obesity. HFD feeding caused pronounced dyslipidemia, hypertension, and insulin resistance (systemic and adipose), as well as induction of inflammatory genes, impairments in vasomotor reactivity to insulin, and atherosclerosis in the coronary arteries. Remarkably, visceral, subcutaneous, and perivascular adipose tissue inflammation (via FACS analysis and RT-PCR) was not increased in OBESE pigs, nor were circulating inflammatory cytokines.
These findings reveal a disconnect between adipose tissue inflammation and cardiometabolic dysfunction induced by Western diet feeding in the Ossabaw pig model.
奥萨巴猪因其体型以及对动脉粥样硬化的易感性等特征，正成为一种颇具吸引力的人类心脏代谢疾病模型。本研究在此探究了该模型中脂肪组织炎症与代谢功能障碍之间的关系。
将年轻的雌性奥萨巴猪分为两组，一组喂食西式高脂饮食（HFD）（n = 4），另一组喂食对照低脂饮食（LFD）（n = 4），为期9个月，然后比较两组的心脏代谢结果和脂肪组织炎症情况。
喂食HFD的“肥胖”猪比喂食LFD的“瘦”猪重2.5倍（P < 0.001），并出现了严重肥胖。喂食HFD导致明显的血脂异常、高血压和胰岛素抵抗（全身性和脂肪性），还诱导了炎症基因的表达，损害了血管对胰岛素的反应性，并导致冠状动脉粥样硬化。值得注意的是，通过流式细胞术分析和逆转录聚合酶链反应发现，“肥胖”猪的内脏、皮下和血管周围脂肪组织炎症并未增加，循环炎症细胞因子也未增加。
这些发现揭示了在奥萨巴猪模型中，西方饮食喂养所诱导的脂肪组织炎症与心脏代谢功能障碍之间并无关联。
