Takahashi Ayako, Inoue Hiroko, Mishima Kenji, Ide Fumio, Nakayama Ryoko, Hasaka Ayaka, Ryo Koufuchi, Ito Yumi, Sakurai Takashi, Hasegawa Yoshinori, Saito Ichiro
Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Japan; Department of Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama, Japan.
PLoS One. 2015 Jan 28;10(1):e0116008. doi: 10.1371/journal.pone.0116008. eCollection 2015.
With the aim of discovering an effective method to treat dry mouth, we analyzed the effects of quercetin on salivary secretion and its mechanism of action. We created a mouse model with impaired salivary secretion by exposure to radiation and found that impaired secretion is suppressed by quercetin intake. Moreover, secretion levels were enhanced in quercetin-fed normal mice. To elucidate the mechanisms of these effects on salivary secretion, we conducted an analysis using mouse submandibular gland tissues, a human salivary gland epithelial cell line (HSY), and mouse aortic endothelial cells (MAECs). The results showed that quercetin augments aquaporin 5 (AQP5) expression and calcium uptake, and suppresses oxidative stress and inflammatory responses induced by radiation exposure, suggesting that quercetin intake may be an effective method to treat impaired salivary secretion.
为了找到治疗口干的有效方法，我们分析了槲皮素对唾液分泌的影响及其作用机制。我们通过辐射创建了唾液分泌受损的小鼠模型，发现摄入槲皮素可抑制分泌受损。此外，喂食槲皮素的正常小鼠的分泌水平有所提高。为了阐明这些对唾液分泌影响的机制，我们使用小鼠颌下腺组织、人唾液腺上皮细胞系（HSY）和小鼠主动脉内皮细胞（MAECs）进行了分析。结果表明，槲皮素可增加水通道蛋白5（AQP5）的表达和钙摄取，并抑制辐射暴露诱导的氧化应激和炎症反应，这表明摄入槲皮素可能是治疗唾液分泌受损的有效方法。
