Yin Hua-Qin, Bi Feng-Li, Gan Feng
School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P.R. China.
Bioconjug Chem. 2015 Feb 18;26(2):243-9. doi: 10.1021/bc500505c. Epub 2015 Jan 28.
A simple, rapid, and inexpensive method for the synthesis of cyclic arginine-glycine-aspartic acid (RGD) peptide conjugated gold nanoclusters (RGD-GNCs) was reported. The nanoclusters were synthesized with chloraurate as precursor and cyclic RGD peptides as both reducing and protecting agent directly under alkali condition, and the whole synthetic process only took 15 min at room temperature. The properties of the nanoclusters were characterized by means of ultraviolet-visible spectra, Fourier transform infrared spectroscopy (FTIR), fluorescence, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The prepared gold nanoclusters were successfully used as a contrast agent in fluorescence imaging of the melanoma A375 cells, which overexpress the integrin αvβ3. The results demonstrated that our nanoclusters possess good biocompatibility, stability, and low toxicity. Moreover, the method is expected to be applicable to the synthesis of nanoclusters conjugated with other biomolecules.
报道了一种简单、快速且廉价的合成环化精氨酸-甘氨酸-天冬氨酸（RGD）肽共轭金纳米簇（RGD-GNCs）的方法。以氯金酸盐为前驱体，环化RGD肽作为还原剂和保护剂，在碱性条件下直接合成纳米簇，整个合成过程在室温下仅需15分钟。通过紫外可见光谱、傅里叶变换红外光谱（FTIR）、荧光、透射电子显微镜（TEM）和X射线光电子能谱（XPS）对纳米簇的性质进行了表征。所制备的金纳米簇成功用作黑色素瘤A375细胞荧光成像的造影剂，该细胞过表达整合素αvβ3。结果表明，我们的纳米簇具有良好的生物相容性、稳定性和低毒性。此外，该方法有望适用于与其他生物分子共轭的纳米簇的合成。
