上尿路尿路上皮癌患者组织和血清微小RNA表达分析
Analysis of tissue and serum microRNA expression in patients with upper urinary tract urothelial cancer.
作者信息
Kriebel Stephanie, Schmidt Doris, Holdenrieder Stefan, Goltz Diane, Kristiansen Glen, Moritz Rudolf, Fisang Christian, Müller Stefan C, Ellinger Jörg
机构信息
Universitätsklinikum Bonn, Klinik und Poliklinik für Urologie und Kinderurologie, Bonn, Germany.
Universitätsklinikum Bonn, Institut für Klinische Chemie und Klinische Pharmakologie, Bonn, Germany.
出版信息
PLoS One. 2015 Jan 28;10(1):e0117284. doi: 10.1371/journal.pone.0117284. eCollection 2015.
INTRODUCTION
MicroRNAs play an important role in many human malignancies; so far, their expression remains to be studied in upper urinary tract urothelial cancer (UUTUC).
MATERIALS AND METHODS
The expression of eleven microRNAs (miR-10a, miR-21, miR-96, miR-135, miR-141, miR-182, miR-200b, miR-205, miR-429, miR-520b, miR-1244) formerly shown to be upregulated in urothelial bladder cancer were studied in corresponding normal and cancerous tissue samples of patients undergoing nephroureterectomy for UUTUC. Upregulated microRNAs were then measured in serum samples of patients with UUTUC and patients with non-malignant urological diseases to evaluate their potential as non-invasive biomarkers for UUTUC.
RESULTS
MicroRNA expression allowed differentiation of normal and cancerous tissue: miR-21, miR-96, miR-135, miR-141, miR-182, miR-205, miR-429 and miR-520b were significantly overexpressed. Furthermore, miR-205 was upregulated in poorly differentiated UUTUC. The analysis of circulating RNA in serum demonstrated an increase of miR-141 in patients with UUTUC; receiver operator characteristic analysis demonstrated an area under the curve of 0.726 for miR-141 as a diagnostic biomarker. Furthermore, we observed lower levels of miR-10a and miR-135 in UUTUC patients.
CONCLUSIONS
MicroRNA expression is altered in UUTUC. The analysis of circulating miR-141 may be useful to identify patients with UUTUC.
引言
微小RNA在许多人类恶性肿瘤中发挥重要作用；迄今为止，它们在上尿路尿路上皮癌（UUTUC）中的表达仍有待研究。
材料与方法
研究了先前在膀胱尿路上皮癌中显示上调的11种微小RNA（miR-10a、miR-21、miR-96、miR-135、miR-141、miR-182、miR-200b、miR-205、miR-429、miR-520b、miR-1244）在接受UUTUC肾输尿管切除术患者的相应正常和癌组织样本中的表达。然后在UUTUC患者和非恶性泌尿系统疾病患者的血清样本中测量上调的微小RNA，以评估它们作为UUTUC非侵入性生物标志物的潜力。
结果
微小RNA表达可区分正常组织和癌组织：miR-21、miR-96、miR-135、miR-141、miR-182、miR-205、miR-429和miR-520b显著过表达。此外，miR-205在低分化UUTUC中上调。血清中循环RNA的分析表明，UUTUC患者中miR-141增加；受试者工作特征分析显示，miR-141作为诊断生物标志物的曲线下面积为0.726。此外，我们观察到UUTUC患者中miR-10a和miR-135水平较低。
结论
UUTUC中微小RNA表达发生改变。循环miR-141的分析可能有助于识别UUTUC患者。
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