Safira Arifia, Rani Cinta Atsa Mahesa, Fikri Faisal, Purnomo Agus, Khairani Shafia, Chhetri Shekhar, Maslamama Salipudin Tasil, Purnama Muhammad Thohawi Elziyad
Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia.
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2023 May;16(5):1084-1091. doi: 10.14202/vetworld.2023.1084-1091. Epub 2023 May 24.
A bone defect is defined as a critically sized autologous bone and a bone gap. Bone grafting is one of the most commonly used surgical methods to enhance bone regeneration in orthopedic procedures. A composite of collagen, hydroxyapatite (HA), and chitosan (Ch) is suitable as a bone matrix and stimulates ossification. This study aimed to evaluate the use of natural HA-Ch composites derived from sea cucumbers and shrimp shells and quantify the levels of cytokines, polymorphonuclear neutrophils (PMNs), serum liver enzymes, calcium, phosphate, and procollagen type 1 N-terminal propeptide (PINP) in albino rats with femoral bone defects.
A total of 48 albino rats with femoral bone defects were divided into 4 groups (n = 12 each): (C-) placebo, (C+) polyethylene glycol, (T1) HA, and (T2) HA-Ch groups. Each group was divided into two subgroups (n = 6 each), with euthanization on 7- and 42-day post-treatment, respectively. Procollagen Type 1 N-terminal propeptide and the cytokines interleukin (IL)-4, IL-6, IL-10, and tumor necrosis factor-alpha were quantified using enzyme-linked immunosorbent assay. Flow cytometry was performed to evaluate PMNs. A clinical chemistry analyzer was used to measure the serum levels of liver enzymes, calcium, and phosphate.
There was a significant decrease in the level of IL-6 on 7 days and in the level of IL-10 on 42 days in the HA-Ch group. The level of PMNs also decreased significantly on 7 and 42 days in the HA-Ch group. Regarding serum liver enzymes, alkaline phosphatase (ALP) levels in the HA-Ch group increased significantly on 42 days. Calcium and phosphate levels increased significantly on 7 and 42 days in the HA and HA-Ch groups, and PINP levels increased significantly on 7 and 42 days in the HA-Ch group.
The HA-Ch composite derived from sea cucumbers and shrimp shells ameliorated femoral bone defects in albino rats. The HA-Ch composite modulated the levels of IL-6, IL-10, PMNs, ALP, calcium, phosphate, and PINP on 7- and 42-day post-treatment.
骨缺损被定义为临界大小的自体骨和骨间隙。骨移植是骨科手术中增强骨再生最常用的手术方法之一。胶原蛋白、羟基磷灰石（HA）和壳聚糖（Ch）的复合材料适合作为骨基质并刺激骨化。本研究旨在评估源自海参和虾壳的天然HA-Ch复合材料的应用，并量化股骨骨缺损白化大鼠体内细胞因子、多形核中性粒细胞（PMN）、血清肝酶、钙、磷和I型前胶原N端前肽（PINP）的水平。
总共48只患有股骨骨缺损的白化大鼠被分为4组（每组n = 12）：（C-）安慰剂组、（C+）聚乙二醇组、（T1）HA组和（T2）HA-Ch组。每组再分为两个亚组（每组n = 6），分别在治疗后7天和42天实施安乐死。使用酶联免疫吸附测定法定量I型前胶原N端前肽以及细胞因子白细胞介素（IL）-4、IL-6、IL-10和肿瘤坏死因子-α。进行流式细胞术以评估PMN。使用临床化学分析仪测量血清肝酶、钙和磷的水平。
HA-Ch组在第7天IL-6水平显著降低，在第42天IL-10水平显著降低。HA-Ch组在第7天和第42天PMN水平也显著降低。关于血清肝酶，HA-Ch组在第42天碱性磷酸酶（ALP）水平显著升高。HA组和HA-Ch组在第7天和第42天钙和磷水平显著升高，HA-Ch组在第7天和第42天PINP水平显著升高。
源自海参和虾壳的HA-Ch复合材料改善了白化大鼠的股骨骨缺损。HA-Ch复合材料在治疗后7天和42天调节了IL-6、IL-10、PMN、ALP、钙、磷和PINP的水平。
