Carvalho Fabiano B, Gutierres Jessié M, Bohnert Crystiani, Zago Adriana M, Abdalla Fátima H, Vieira Juliano M, Palma Heloisa E, Oliveira Sara M, Spanevello Roselia M, Duarte Marta M, Lopes Sonia T A, Aiello Graciane, Amaral Marta G, Pippi Ney Luis, Andrade Cinthia M
Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Setor de Bioquímica e Estresse Oxidativo do Laboratório de Terapia Celular, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria RS 97105-900, Brasil.
Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Química, Centro de Ciências Naturais e Exatas; Universidade Federal de Santa Maria, Santa Maria RS 97105-900, Brasil.
J Nutr Biochem. 2015 Apr;26(4):378-90. doi: 10.1016/j.jnutbio.2014.11.006. Epub 2014 Dec 15.
The aim of this study was to investigate the protective effect of anthocyanins (ANT) on oxidative and inflammatory parameters, as well as ion pump activities, in the pons of rats experimentally demyelinated with ethidium bromide (EB). Rats were divided in six groups: control, ANT 30 mg/kg, ANT 100 mg/kg, EB (0.1%), EB plus ANT 30 mg/kg and EB plus ANT 100 mg/kg. The EB cistern pons injection occurred on the first day. On day 7, there was a peak in the demyelination. During the 7 days, the animals were treated once per day with vehicle or ANT. It was observed that demyelination reduced Na(+),K(+)-ATPase and Ca(2+)-ATPase activities and increased 4-hydroxynonenal, malondialdehyde, protein carbonyl and NO2plus NO3 levels. In addition, a depletion of glutathione reduced level/nonprotein thiol content and a decrease in superoxide dismutase activity were also seen. The dose of 100 mg/kg showed a better dose-response to the protective effects. The demyelination did not affect the neuronal viability but did increase the inflammatory infiltrate (myeloperoxidase activity) followed by an elevation in interleukin (IL)-1β, IL-6, tumor necrosis factor-α and interferon-γ levels. ANT promoted a reduction in cellular infiltration and proinflammatory mediators. Furthermore, ANT restored the levels of IL-10. Luxol fast blue staining confirmed the loss of myelin in the EB group and the protective effect of ANT 100 mg/kg. In conclusion, this study was the first to show that ANT are able to restore ion pump activities and protect cellular components against the inflammatory and oxidative damages induced by demyelination.
本研究旨在探讨花青素（ANT）对用溴化乙锭（EB）进行实验性脱髓鞘的大鼠脑桥氧化和炎症参数以及离子泵活性的保护作用。将大鼠分为六组：对照组、ANT 30 mg/kg组、ANT 100 mg/kg组、EB（0.1%）组、EB加ANT 30 mg/kg组和EB加ANT 100 mg/kg组。第一天进行EB脑池脑桥注射。第7天，脱髓鞘达到高峰。在这7天里，动物每天用溶剂或ANT治疗一次。观察到脱髓鞘降低了Na(+)、K(+)-ATP酶和Ca(2+)-ATP酶的活性，并增加了4-羟基壬烯醛、丙二醛、蛋白质羰基和NO2加NO3的水平。此外，还观察到谷胱甘肽水平降低/非蛋白质巯基含量减少以及超氧化物歧化酶活性下降。100 mg/kg的剂量对保护作用显示出更好的剂量反应。脱髓鞘不影响神经元活力，但确实增加了炎症浸润（髓过氧化物酶活性），随后白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α和干扰素-γ水平升高。ANT促进了细胞浸润和促炎介质的减少。此外，ANT恢复了IL-10的水平。Luxol固蓝染色证实了EB组髓磷脂的损失以及ANT 100 mg/kg的保护作用。总之，本研究首次表明ANT能够恢复离子泵活性，并保护细胞成分免受脱髓鞘诱导的炎症和氧化损伤。
