Wang Qi-Xian, Zhu You-Qing, Zhang Hu, Xiao Jun
Department of Gastroenterology and Laboratory of Gastroenterology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China.
Cell Physiol Biochem. 2015;35(3):933-44. doi: 10.1159/000369750. Epub 2015 Jan 30.
BACKGROUND/AIMS: Aberrant microRNA expression has the potential to be used for early diagnosis of gastric cancer or to predict survival and treatment response. This study performed a systematic review and meta-analysis of altered miRNAs in gastric cancer in order to assess the use of miRNAs as novel biomarkers for early detection and prognosis prediction of gastric cancer.
We retrieved published articles from the PubMed online database and obtained different sets of data on miRNAs expression profiling in gastric cancer and highlighted the most frequently dysregulated miRNAs in gastric cancer. We then extracted studies that used quantitative RT-PCR and then pooled them together by using meta-disc software (version 1.4).
We found that there were 47 aberrantly expressed miRNAs in gastric cancer (29 up-regulated and 18 down-regulated) that were most frequently reported in the literature. In publications that provided information on specific miRNA expression vs. diagnostic value, the pooled data showed good sensitivity and specificity as well as high levels of overall accuracy. However, specimen types could be a factor that introduces substantial heterogeneity. Published studies also showed association of altered miRNA expression with clinicopathological data from gastric cancer patients.
Thus, various miRNAs are differentially expressed in gastric cancer and some of them could be further evaluated as biomarkers for early diagnosis of gastric cancer and prediction of prognosis or treatment response.
背景/目的：异常的微小RNA表达有潜力用于胃癌的早期诊断或预测生存及治疗反应。本研究对胃癌中改变的微小RNA进行了系统评价和荟萃分析，以评估微小RNA作为胃癌早期检测和预后预测新生物标志物的应用。
我们从PubMed在线数据库检索已发表的文章，并获得了关于胃癌中微小RNA表达谱的不同数据集，突出了胃癌中最常失调的微小RNA。然后我们提取了使用定量逆转录聚合酶链反应的研究，并使用Meta-Disc软件（1.4版）将它们汇总在一起。
我们发现胃癌中有47种异常表达的微小RNA（29种上调和18种下调），这些在文献中报道最为频繁。在提供特定微小RNA表达与诊断价值信息的出版物中，汇总数据显示出良好的敏感性和特异性以及较高的总体准确性水平。然而，标本类型可能是引入大量异质性的一个因素。已发表的研究还显示了微小RNA表达改变与胃癌患者临床病理数据之间的关联。
因此，多种微小RNA在胃癌中差异表达，其中一些可进一步评估作为胃癌早期诊断及预后或治疗反应预测的生物标志物。
