de Lucena Maurilio Toscano, de Melo Júnior Mário Ribeiro, de Melo Lira Mariana Montenegro, de Castro Célia Maria Machado Barbosa, Cavalcanti Leonardo Aguiar, de Menezes Mariana Arruda, Pinto Flávia Cristina Morone, de Andrade Aguiar José Lamartine
Department of Surgery, Center for Health Sciences, Federal University of Pernambuco, UFPE, Recife, Brazil.
J Mater Sci Mater Med. 2015 Feb;26(2):82. doi: 10.1007/s10856-015-5410-x. Epub 2015 Jan 30.
The development of a skin substitute suitable for immediately performing the function of the lost dermis and epidermis could result in a positive impact on the treatment of patients with extensive skin lesions. A biopolymer film was applied to skin wounds to investigate the biocompatibility and cutaneous reaction and to test its activity as a mechanical barrier and conductor in the healing process. Forty Wistar rats of both sexes were used in the present study. Two excisions were performed in the dorsal part of the skin flaps. The polysaccharide film was applied over one of the incisions and other incision was washed with saline. The time spent for complete healing of both lesions was virtually the same in both groups, during 21 days of observation. The film remained attached to the bed of the exposed wound for an average period of 6 days. There were no statistically significant differences with regard to lesion measurement area at assessment times of 2nd, 7th and 14th postoperative days. At day 21, the scar area showed a significant difference (0.0229). After 40 days, all wounds were completely healed. No statistically significant differences were found between the histological parameters assessed in the experimental and control groups. The cellulosic polysaccharide film integrated well with the tissue showing high biocompatibility and low skin reactivity.
开发一种适合立即发挥缺失真皮和表皮功能的皮肤替代品，可能会对广泛皮肤损伤患者的治疗产生积极影响。将一种生物聚合物薄膜应用于皮肤伤口，以研究其生物相容性和皮肤反应，并测试其在愈合过程中作为机械屏障和导体的活性。本研究使用了40只雌雄皆有的Wistar大鼠。在皮瓣的背部进行了两处切口。将多糖薄膜覆盖在其中一个切口上，另一个切口用盐水冲洗。在21天的观察期内，两组伤口完全愈合所需的时间几乎相同。该薄膜在暴露伤口的创面上平均附着6天。在术后第2天、第7天和第14天的评估时间点，病变测量面积没有统计学上的显著差异。在第21天，瘢痕面积显示出显著差异（0.0229）。40天后，所有伤口完全愈合。在实验组和对照组评估的组织学参数之间未发现统计学上的显著差异。纤维素多糖薄膜与组织良好整合，显示出高生物相容性和低皮肤反应性。
