Universidade Federal Rural de Pernambuco - Department of Animal Morphology and Physiology - Graduate Program in Animal Bioscience - Recife (PE) - Brazil.
Universidade Federal de Alagoas - Maceió (AL) - Brazil.
Acta Cir Bras. 2024 Oct 25;39:e399024. doi: 10.1590/acb399024. eCollection 2024.
To describe the effects of melatonin associated with bacterial cellulose-based hydrogel on healing of skin wounds in diabetic rats.
Streptozotocin was used to induce diabetes in Wistar rats. After wound induction, animals were randomly divided into groups GC, GDCC, GDCB, and GDMCB. Animals were evaluated in days 3, 7, and 14 for the following variables: glycemic levels, histopathological and histochemical analyses, healing rate, morphometry and C-reactive protein.
There was no change in glycemic levels in the diabetic animals as a result of the treatments; histopathological analyses showed better healing in GDCB and GDMCB groups, as well as histochemistry; at day 14, the highest healing rate was observed in animals from the GDMCB group, reaching almost 100%; morphometry revealed a significant increase of fibroblasts and reduction of macrophages and blood vessels in lesions treated with bacterial cellulose associated or not with melatonin when compared to the other experimental groups. There was also an increase in C-reactive protein in GDCC group at day 14.
Bacterial cellulose-based dressings associated with systemic melatonin showed beneficial results in experimentally induced wounds in diabetic rats, favoring the healing process.
描述与细菌纤维素基水凝胶相关的褪黑素对糖尿病大鼠皮肤伤口愈合的影响。
链脲佐菌素用于诱导 Wistar 大鼠糖尿病。诱导伤口后，动物随机分为 GC、GDCC、GDCB 和 GDMCB 组。在第 3、7 和 14 天评估以下变量：血糖水平、组织病理学和组织化学分析、愈合率、形态计量学和 C 反应蛋白。
治疗并未改变糖尿病动物的血糖水平；组织病理学分析显示 GDCB 和 GDMCB 组的愈合情况更好，组织化学分析也是如此；在第 14 天，GDMCB 组的动物观察到最高的愈合率，接近 100%；形态计量学显示，与其他实验组相比，用细菌纤维素联合或不联合褪黑素处理的病变中，成纤维细胞显著增加，巨噬细胞和血管减少。在第 14 天，GDCC 组的 C 反应蛋白也增加。
与系统褪黑素相关的细菌纤维素敷料在实验诱导的糖尿病大鼠伤口中显示出有益的结果，有利于愈合过程。
