Shi Shuang, Bao Zhi-fan, Chen Xu, Zhang Dan-dan
Department of Pediatric Dentistry, School of Stomatology, China Medical University,Liaoning Research Institute of Stomatology. Shenyang 110002, Liaoning Province, China.E-mail:
Shanghai Kou Qiang Yi Xue. 2014 Dec;23(6):681-4.
To compare the cytotoxicity of a novel endodontic treatment material iRoot BP Plus and mineral trioxide aggregate (MTA) to human gingival fibroblasts in vitro.
Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes (no dilution, 1:2 dilution, and 1:5 dilution). The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37 degrees centigrade. The proliferation rate was evaluated using methylthiazol tetrazolium (MTT) assay. Cell relative growth rates were presented as x±s. The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package.
Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The cytotoxicity grade of both materials was 0 or 1 (no cytotoxicity). There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times (F(concentration×time×material)=1.393, P=0.256).
Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.
在体外比较新型根管治疗材料iRoot BP Plus和三氧化矿物凝聚体（MTA）对人牙龈成纤维细胞的细胞毒性。
将培养的人牙龈成纤维细胞暴露于多种浓度的材料洗脱液（未稀释、1:2稀释和1:5稀释）中。将测试材料样本在37摄氏度下于培养基中浸泡并孵育1、3或7天。使用噻唑蓝（MTT）法评估增殖率。细胞相对生长率以x±s表示。使用SPSS 20.0软件包通过析因设计方差分析对数据进行统计学分析。
不同浓度的iRoot BP Plus和MTA洗脱液的细胞相对生长率在77.31%至113.82%之间。两种材料的细胞毒性等级均为0或1（无细胞毒性）。在不同洗脱时间下，不同浓度的iRoot BP Plus和MTA洗脱液的相对生长率无显著差异（F（浓度×时间×材料）=1.393，P=0.256）。
iRoot BP Plus和MTA均表现出极低水平的细胞毒性。
