Tian Jun, Zhang Yuanhe, Lai Zhihui, Li Mengjie, Huang Yu, Jiang Hongwei, Wei Xi
Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, P.R. China.
Instrumental Analysis and Research Center, Sun Yat-sen University, Guangzhou, P.R. China; School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
J Endod. 2017 Jan;43(1):163-168. doi: 10.1016/j.joen.2016.10.011. Epub 2016 Dec 6.
This study evaluated how exposing the novel calcium silicate nanoparticulate bioceramic iRoot BP Plus (Innovative Bioceramix, Vancouver, Canada) to an acidic environment affects ion release from this material and alters MC3T3-E1 preosteoblast viability on and attachment to this material. These factors were compared against those of ProRoot MTA under similar conditions.
Each material was exposed to phosphate-buffered saline (pH = 7.4) or butyric acid (pH = 5.4) for 5 days. Trace metal elements within the 2 materials and released ions were identified using inductively coupled plasma optical emission spectroscopy. The microstructures and elemental compositions of MTA and iRoot BP Plus after treatment with butyric acid were determined using scanning electron microscopy with an energy-dispersive X-ray spectrometer. Furthermore, the viability of MC3T3-E1 cells on and their levels of attachment to the materials after the butyric acid treatment were compared.
iRoot BP Plus contained fewer toxic metal elements than MTA. Under acidic conditions, both materials displayed similar ion release abilities, with increased release of Si and Ca ions. Substantial changes in microstructure, including reduced apatite formation, were observed for both materials after exposure to acidic pH. Furthermore, exposing iRoot BP Plus and MTA to an acidic environment increased and decreased MC3T3-E1 cell viability on these materials, respectively. MC3T3-E1 cell attachment to both materials was not significantly affected by acidic pH.
iRoot BP Plus seems more biologically appropriate for application in an inflamed acidic environment than ProRoot MTA.
本研究评估了将新型硅酸钙纳米颗粒生物陶瓷iRoot BP Plus（加拿大温哥华Innovative Bioceramix公司）置于酸性环境中如何影响该材料的离子释放，以及如何改变MC3T3-E1前成骨细胞在该材料上的活力和附着情况。在相似条件下，将这些因素与ProRoot MTA的相关因素进行了比较。
将每种材料置于磷酸盐缓冲盐水（pH = 7.4）或丁酸（pH = 5.4）中5天。使用电感耦合等离子体发射光谱法鉴定两种材料中的痕量金属元素和释放的离子。使用配备能量色散X射线光谱仪的扫描电子显微镜测定用丁酸处理后MTA和iRoot BP Plus的微观结构和元素组成。此外，比较了丁酸处理后MC3T3-E1细胞在材料上的活力及其附着水平。
iRoot BP Plus含有的有毒金属元素比MTA少。在酸性条件下，两种材料表现出相似的离子释放能力，硅和钙离子的释放增加。暴露于酸性pH值后，两种材料均观察到微观结构的显著变化，包括磷灰石形成减少。此外，将iRoot BP Plus和MTA置于酸性环境中分别增加和降低了MC3T3-E1细胞在这些材料上的活力。酸性pH值对MC3T3-E1细胞与两种材料的附着没有显著影响。
与ProRoot MTA相比，iRoot BP Plus在炎症酸性环境中的应用似乎在生物学上更合适。
