Bry K J K, Jacobsson B, Nilsson S, Bry K
Department of Pediatrics, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, Sweden.
Department of Obstetrics and Gynecology, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, Sweden.
Acta Paediatr. 2015 Jun;104(6):575-80. doi: 10.1111/apa.12947. Epub 2015 Mar 27.
The aim of this study was to determine whether the concentration of cytokines in the gastric fluid at birth was associated with chorioamnionitis or funisitis and with the white blood cell counts of very premature newborns.
We retrieved gastric fluid from 27 preterm infants with a gestational age of <29 weeks within 1 h of birth and used enzyme-linked immunosorbent assay to measure the concentrations of interleukin (IL)-1beta, epithelial cell-derived neutrophil-activating peptide (ENA)-78, IL-8 and growth-related oncogene (Gro)-alpha. The presence of histologic chorioamnionitis or funisitis in the placentas and the highest white blood cell count of the infants during the first week of life were compared to the cytokine concentrations.
Gastric fluid concentrations of IL-1beta, ENA-78, IL-8 and Gro-alpha were strongly associated with chorioamnionitis and funisitis. In addition, chorioamnionitis and funisitis and gastric aspirate cytokine levels were associated with the highest white blood cell counts of the infants during the first week of life.
This study suggests that levels of inflammatory cytokines in the gastric fluid of premature infants at birth can be used to assess the exposure of the infants to antenatal inflammation.
本研究旨在确定出生时胃液中细胞因子的浓度是否与绒毛膜羊膜炎或脐带炎以及极早产儿的白细胞计数相关。
我们在出生后1小时内从27例胎龄小于29周的早产儿中获取胃液，并使用酶联免疫吸附测定法测量白细胞介素（IL）-1β、上皮细胞衍生的中性粒细胞激活肽（ENA）-78、IL-8和生长相关癌基因（Gro）-α的浓度。将胎盘组织学绒毛膜羊膜炎或脐带炎的存在情况以及婴儿出生后第一周的最高白细胞计数与细胞因子浓度进行比较。
IL-1β、ENA-78、IL-8和Gro-α的胃液浓度与绒毛膜羊膜炎和脐带炎密切相关。此外，绒毛膜羊膜炎、脐带炎和胃吸出物细胞因子水平与婴儿出生后第一周的最高白细胞计数相关。
本研究表明，早产儿出生时胃液中炎症细胞因子水平可用于评估婴儿产前炎症暴露情况。
