a Department of Pediatrics, Division of Neonatology , University of Florida , Gainesville , USA.
b College of Nursing , University of Florida , Gainesville , USA.
Gut Microbes. 2019;10(3):289-297. doi: 10.1080/19490976.2018.1520578. Epub 2018 Nov 7.
The purpose of this prospective cross-sectional cohort pilot study is to explore the initial microbial community of gastric aspirate fluid as collected immediately after birth and its relationships with mode of delivery and preterm birth. Twenty-nine gastric aspirate samples collected immediately after birth from infants born between 24-40 weeks gestation were analyzed for microbial composition. Total microbial content was low in many samples, with a substantial number sharing taxonomic composition with negative controls. qPCR targeting the 16S rRNA gene showed that infants delivered vaginally had a higher microbial load than infants delivered by C-section. Some pre-term samples showed high relative abundance of genus , consistent with previous literature that has implicated infections with this taxon as a potential cause of pre-term birth. Vaginally born term infant samples, by contrast, had significantly higher levels of genus with the most dominant species. Microbial evaluation showed that vaginally born term infant gastric aspirate samples had higher levels of lactobacilli than pre-terms. Samples from many infants had low microbial load near the edge of the detection limit.
本前瞻性横断面队列研究旨在探索出生后立即采集的胃抽吸液中的初始微生物群落及其与分娩方式和早产的关系。对 29 名在 24-40 周妊娠期间出生的婴儿出生后立即采集的胃抽吸样本进行了微生物组成分析。许多样本中的总微生物含量较低，与阴性对照具有相同分类组成的样本数量很多。针对 16S rRNA 基因的 qPCR 显示，阴道分娩的婴儿比剖宫产分娩的婴儿具有更高的微生物负荷。一些早产样本显示属的相对丰度较高，这与先前的文献一致，该文献表明该分类群的感染可能是早产的潜在原因。相比之下，阴道分娩的足月婴儿样本中属的水平明显较高，其中最主要的物种是。微生物评估表明，阴道分娩的足月婴儿胃抽吸样本中的乳杆菌水平高于早产儿。许多婴儿的样本微生物负荷接近检测限的边缘，水平较低。
