Ludden C, Brennan G, Morris D, Austin B, O'Connell B, Cormican M
Antimicrobial Resistance and Microbial Ecology (ARME) Group,School of Medicine, National University of Ireland,Galway,Ireland.
National MRSA Reference Laboratory,St James's Hospital,Dublin,Ireland.
Epidemiol Infect. 2015 Oct;143(14):2985-8. doi: 10.1017/S0950268815000072. Epub 2015 Feb 2.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major public health concern associated with residence in a long-term care facility (LTCF). The aim of this prospective study was to characterize MRSA isolated from residents over a 1-year period and their physical environment over a 2-year period. MRSA was recovered from 17/64 residents (R) of a LTCF and from 42 environmental (E) sites. All isolates carried the mecA gene and lacked the mecC and Panton-Valentine leukocidin (PVL) genes. Thirteen spa types were identified with t032 being the most frequent (41% of total; n = 8R, 16E), followed by t727 (22% of total; n = 13E), and t8783 (10% of total; n = 6E). Five spa types were each represented by single isolates. Thirty-nine isolates were of spa types associated with the multilocus sequence type ST22 (t032, 41%; spa-CC22, 68%) and reflect the predominance of ST22 in Irish hospitals. The uncommon spa types t727, t8783, t1372, t3130, t10038 were present in the environment but not detected in residents and are infrequently observed in Ireland.
耐甲氧西林金黄色葡萄球菌（MRSA）是与长期护理机构（LTCF）居住相关的一个主要公共卫生问题。这项前瞻性研究的目的是对在1年期间从居民中分离出的MRSA及其在2年期间的物理环境进行特征描述。从一家长期护理机构的17/64名居民（R）和42个环境（E）位点中分离出了MRSA。所有分离株都携带mecA基因，且缺乏mecC和杀白细胞素（PVL）基因。鉴定出了13种spa型，其中t032最为常见（占总数的41%；n = 8名居民，16个环境位点），其次是t727（占总数的22%；n = 13个环境位点）和t8783（占总数的10%；n = 6个环境位点）。有5种spa型各由单一分离株代表。39株分离株属于与多位点序列类型ST22相关的spa型（t032，41%；spa-CC22，68%），反映了ST22在爱尔兰医院中的优势地位。不常见的spa型t727、t8783、t1372、t3130、t10038存在于环境中，但在居民中未检测到，在爱尔兰也很少观察到。
