Grassmann J P, Schneppendahl J, Hakimi A R, Herten M, Betsch M, Lögters T T, Thelen S, Sager M, Wild M, Windolf J, Jungbluth P, Hakimi M
Heinrich Heine University Hospital D, uesseldorf, Department of Trauma and Hand Surgery, Moorenstrasse 5, D-40225 Duesseldorf, Germany.
J Orthop Res. 2015 Apr;33(4):513-20. doi: 10.1002/jor.22805.
Besides the use of autologous bone grafting several osteoconductive and osteoinductive methods have been reported to improve bone healing. However, persistent non-union occurs in a considerable number of cases and compromised angiogenesis is suspected to impede bone regeneration. Hyperbaric oxygen therapy (HBO) improves angiogenesis. This study evaluates the effects of HBO on bone defects treated with autologous bone grafting in a bone defect model in rabbits. Twenty-four New-Zealand White Rabbits were subjected to a unilateral critical sized diaphyseal radius bone defect and treated with autologous cancellous bone transplantation. The study groups were exposed to an additional HBO treatment regimen. Bone regeneration was evaluated radiologically and histologically at 3 and 6 weeks, angiogenesis was assessed by immunohistochemistry at three and six weeks. The additional administration of HBO resulted in a significantly increased new bone formation and angiogenesis compared to the sole treatment with autologous bone grafting. These results were apparent after three and six weeks of treatment. The addition of HBO therapy to autologous bone grafts leads to significantly improved bone regeneration. The increase in angiogenesis observed could play a crucial role for the results observed.
除了使用自体骨移植外，还报道了几种骨传导和骨诱导方法来促进骨愈合。然而，相当数量的病例中仍会出现持续性骨不连，并且怀疑血管生成受损会阻碍骨再生。高压氧疗法（HBO）可改善血管生成。本研究在兔骨缺损模型中评估了HBO对自体骨移植治疗的骨缺损的影响。24只新西兰白兔接受单侧临界尺寸的骨干桡骨骨缺损，并接受自体松质骨移植治疗。研究组接受额外的HBO治疗方案。在3周和6周时通过放射学和组织学评估骨再生，在3周和6周时通过免疫组织化学评估血管生成。与仅用自体骨移植治疗相比，额外给予HBO导致新骨形成和血管生成显著增加。这些结果在治疗3周和6周后很明显。自体骨移植联合HBO疗法可显著改善骨再生。观察到的血管生成增加可能对观察到的结果起关键作用。
