Kremer Dario, Košir Iztok Jože, Končić Marijana Zovko, Čerenak Andreja, Potočnik Tanja, Srečec Siniša, Randić Marko, Kosalec Ivan
Faculty of Pharmacy and Biochemistry University of Zagreb, A. Kovačića 1, HR-10000 Zagreb, Croatia.
Curr Drug Targets. 2015;16(14):1623-33. doi: 10.2174/1389450116666150202161926.
Satureja montanaL. and S. subspicata Vis. (Lamiaceae) are used for centuries in traditional medicine of Balcanic people in the healing of the lymphatic nodule and respiratory system inflammation. In this paper the amount of total phenols and flavonoids (analyzed by UV/Vis spectrophotometry), phenolic compounds profile (analyzed by HPLC), antimicrobial and antioxidant activities were studied in samples collected in seven per species populations of S. montanaand S. subspicatain Croatia. Eight phenolic compounds (rutin, quercetin, caffeic, p-coumaric, ellagic, protocatehuic, rosmarinic, and syringic acid) were identified and quantified using HPLC in methanolic and ethanolic extracts. Results showed that both species contained polyphenolics and other antioxidant compounds with chelating and radical-scavenging properties. The extracts prepared from both species showed broad spectrum of antimicrobial activity on in vitrotested microbial species (Staphylococcus aureus, Escherichia coli, Candida albicans, C. dubliniensis, C. krusei, C. glabrata, C. parapsilosis, and Microsporum gypseum).
山地鼠尾草(Satureja montana L.)和亚穗状鼠尾草(S. subspicata Vis.)(唇形科)在巴尔干地区的传统医学中用于治疗淋巴结和呼吸系统炎症已有数百年历史。本文对克罗地亚境内山地鼠尾草和亚穗状鼠尾草各七个种群采集的样本进行了研究,分析了总酚和黄酮类化合物的含量(通过紫外/可见分光光度法)、酚类化合物谱(通过高效液相色谱法)、抗菌和抗氧化活性。使用高效液相色谱法对甲醇提取物和乙醇提取物中的八种酚类化合物(芦丁、槲皮素、咖啡酸、对香豆酸、鞣花酸、原儿茶酸、迷迭香酸和丁香酸)进行了鉴定和定量。结果表明,这两个物种均含有具有螯合和自由基清除特性的多酚类及其他抗氧化化合物。从这两个物种制备的提取物对体外测试的微生物物种(金黄色葡萄球菌、大肠杆菌、白色念珠菌、都柏林念珠菌、克柔念珠菌、光滑念珠菌、近平滑念珠菌和石膏样小孢子菌)均表现出广谱抗菌活性。