Vicario A, Sergo V, Toffoli G, Bonifacio A
Department of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127 Trieste, TS, Italy; Experimental and Clinical Pharmacology Division, Department of Translational Research, IRCCS-National Cancer Institute, Aviano, PN, Italy.
Department of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127 Trieste, TS, Italy.
Colloids Surf B Biointerfaces. 2015 Mar 1;127:41-6. doi: 10.1016/j.colsurfb.2015.01.023. Epub 2015 Jan 22.
The development of nanotechnological devices and their clinical application in medicine has become increasingly important, especially in the context of targeted and personalized therapy. This is particularly important in cancer therapy, where antitumor drugs are highly cytotoxic and often exert their therapeutic effect at concentrations close to systemic toxicity. In the last years a growing number of studies has started to report the use of plasmonic nanoprobes in the field of theranostics, broadening the application of vibrational spectroscopies like Raman scattering and surface enhanced Raman scattering (SERS) in biomedicine. The present work aims to identify and characterize the vibrational profiles of a widely used anticancer drug, irinotecan (CPT-11). With a rational approach, SERS experiments have been performed on this analyte employing both Au and Ag colloids, starting from simple aqueous solutions up to albumin mixtures. A major step forward for drug detection in albumin solutions has been taken with the adoption of a simple deproteinization strategy, and a two-in-one-step separation and identification by coupling thin layer chromatography, TLC, with SERS (TLC-SERS). The latter has revealed to be a valid system for protein separation and simultaneous analyte detection, showing a potential to become an innovative, sensitive and low cost method for antineoplastic drug profiling in patients' body fluids.
纳米技术设备的发展及其在医学临床中的应用变得越来越重要，尤其是在靶向和个性化治疗的背景下。这在癌症治疗中尤为重要，因为抗肿瘤药物具有高度细胞毒性，且往往在接近全身毒性的浓度下发挥治疗作用。近年来，越来越多的研究开始报道等离子体纳米探针在治疗诊断学领域的应用，拓宽了拉曼散射和表面增强拉曼散射（SERS）等振动光谱在生物医学中的应用。本工作旨在识别和表征一种广泛使用的抗癌药物伊立替康（CPT-11）的振动谱。通过合理的方法，从简单的水溶液到白蛋白混合物，使用金和银胶体对该分析物进行了SERS实验。通过采用简单的脱蛋白策略以及将薄层色谱（TLC）与SERS联用（TLC-SERS）进行两步分离和鉴定，在白蛋白溶液中的药物检测方面向前迈出了重要一步。后者已被证明是一种有效的蛋白质分离和同时分析物检测系统，显示出有潜力成为一种用于患者体液中抗肿瘤药物分析的创新、灵敏且低成本的方法。
