Du Ran, Yan Jianbin, Li Shizhong, Zhang Lei, Zhang Sandra, Li Jihong, Zhao Gang, Qi Panlu
Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.
Beijing Engineering Research Center for Biofuels, Tsinghua University, Beijing, 100084 China.
Biotechnol Biofuels. 2015 Jan 23;8(1):10. doi: 10.1186/s13068-014-0186-7. eCollection 2015.
Natural bacterial consortia are considered a promising solution for one-step production of ethanol from lignocellulose because of their adaptation to a wide range of natural lignocellulosic substrates and their capacity for efficient cellulose degradation. However, their low ethanol conversion efficiency has greatly limited the development and application of natural bacterial consortia.
In the present study, we analyzed 16 different natural bacterial consortia from a variety of habitats in China and found that the HP consortium exhibited relatively high ethanol production (2.06 g/L ethanol titer from 7 g/L α-cellulose at 55°C in 6 days). Further studies showed that Pseudoxanthomonas taiwanensis played an important role in the high ethanol productivity of HP and that this strain effectively boosted the ethanol production of various other natural bacterial consortia. Finally, we developed a new consortium, termed HPP, by optimizing the proportion of P. taiwanensis in the HP consortium to achieve the highest ethanol production reported for natural consortia. The ethanol conversion ratio reached 78%, with ethanol titers up to 2.5 g/L.
In the present study, we found a natural bacterial consortium with outstanding ethanol production performance, and revealed an efficient method with potentially broad applicability for further improving the ethanol production of natural bacterial consortia.
天然细菌群落被认为是从木质纤维素一步生产乙醇的一种有前景的解决方案，因为它们能适应多种天然木质纤维素底物，且具有高效降解纤维素的能力。然而，它们较低的乙醇转化效率极大地限制了天然细菌群落的开发和应用。
在本研究中，我们分析了来自中国多种生境的16种不同的天然细菌群落，发现HP群落表现出相对较高的乙醇产量（在55℃下6天内从7g/Lα-纤维素产生2.06g/L乙醇滴度）。进一步研究表明，台湾假黄单胞菌在HP的高乙醇生产率中起重要作用，且该菌株有效提高了各种其他天然细菌群落的乙醇产量。最后，我们通过优化台湾假黄单胞菌在HP群落中的比例，开发了一种新的群落，称为HPP，以实现天然群落中报道的最高乙醇产量。乙醇转化率达到78%，乙醇滴度高达2.5g/L。
在本研究中，我们发现了一种具有出色乙醇生产性能的天然细菌群落，并揭示了一种具有潜在广泛适用性的有效方法，可进一步提高天然细菌群落的乙醇产量。
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