Biotechnology and Bioinformatics, NIIT University, Neemrana, Rajasthan 301705, India.
Biotechnology and Bioinformatics, NIIT University, Neemrana, Rajasthan 301705, India.
Carbohydr Polym. 2015 Dec 10;134:761-6. doi: 10.1016/j.carbpol.2015.08.072. Epub 2015 Aug 28.
A microbial consortium, designated Con R, was established by successive sub-cultivation which can degrade 83% of filter paper after 15 days of incubation over control. Among the 14 bacterial isolates obtained from Con R, only bacterial isolate (R-28) was capable of degrading filter paper. Based on 16S rRNA gene sequence, R-28 was identified as Pseudoxanthomonas sp R-28. After 5 days of incubation, degradation efficiencies of Pseudoxanthomonas sp R-28 on filter paper and pure cellulosic waste were 96% and 95% respectively as compared to control. Pseudoxanthomonas sp R-28 also degraded 60% of non-pretreated rice straw after 7 days as compared to control. The degradation kinetics through a modified logistic model showed high correlation coefficient (R(2)) of 0.965 and 0.665 for cellulosic and rice straw waste degradation respectively. Micro scale structural analysis showed the development of fissures and gaps over time which further supported the degradation potential of Pseudoxanthomonas sp R-28.
一个微生物联合体，被命名为 Con R，通过连续的亚培养建立，在 15 天的孵育后，对照中可降解 83%的滤纸。从 Con R 中获得的 14 个细菌分离株中，只有细菌分离株（R-28）能够降解滤纸。基于 16S rRNA 基因序列，R-28 被鉴定为 Pseudoxanthomonas sp R-28。经过 5 天的培养，与对照相比，Pseudoxanthomonas sp R-28 对滤纸和纯纤维素废物的降解效率分别为 96%和 95%。与对照相比，Pseudoxanthomonas sp R-28 还在 7 天内将未经预处理的水稻秸秆降解了 60%。通过修正的逻辑斯蒂模型进行的降解动力学研究显示，纤维素和水稻秸秆废物降解的高相关系数（R(2)）分别为 0.965 和 0.665。微观结构分析表明，随着时间的推移，裂缝和间隙的发展进一步支持了 Pseudoxanthomonas sp R-28 的降解潜力。
