Department of Ophthalmology, UPMC Eye Center, Ophthalmology and Visual Science Research Center, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Invest Ophthalmol Vis Sci. 2014 Jan 2;55(1):1-15. doi: 10.1167/iovs.13-12724.
To measure high-resolution eye-specific displacements and deformations induced within the human LC microstructure by an acute increase in IOP.
Six eyes from donors aged 23 to 82 were scanned using second harmonic-generated (SHG) imaging at various levels of IOP from 10 to 50 mm Hg. An image registration technique was developed, tested, and used to find the deformation mapping between maximum intensity projection images acquired at low and elevated IOP. The mappings were analyzed to determine the magnitude and distribution of the IOP-induced displacements and deformations and contralateral similarity.
Images of the LC were obtained and the registration technique was successful. IOP increases produced substantial, and potentially biologically significant, levels of in-plane LC stretch and compression (reaching 10%-25% medians and 20%-30% 75th percentiles). Deformations were sometimes highly focal and concentrated in regions as small as a few pores. Regions of largest displacement, stretch, compression, and shear did not colocalize. Displacements and strains were not normally distributed. Contralateral eyes did not always have more similar responses to IOP than unrelated eyes. Under elevated IOP, some LC regions were under bi-axial stretch, others under bi-axial compression.
We obtained eye-specific measurements of the complex effects of IOP on the LC with unprecedented resolution in uncut and unfixed human eyes. Our technique was robust to electronic and speckle noise. Elevated IOP produced substantial in-plane LC stretch and compression. Further research will explore the effects of IOP on the LC in a three-dimensional framework.
测量人眼 LC 微观结构在眼内压（IOP）急性升高时引起的高分辨率眼特异性位移和变形。
使用二次谐波产生（SHG）成像技术，对来自 23 岁至 82 岁供体的 6 只眼睛进行扫描，IOP 范围从 10 至 50mmHg。开发了一种图像配准技术，并对其进行了测试，用于在低 IOP 和高 IOP 获得的最大强度投影图像之间找到变形映射。分析映射图以确定 IOP 诱导的位移和变形的大小和分布以及对侧相似性。
获得了 LC 的图像，并且配准技术是成功的。IOP 增加导致了可观的、潜在具有生物学意义的 LC 平面内拉伸和压缩（中位数达到 10%-25%，75%分位数达到 20%-30%）。变形有时非常集中，局限于几个孔大小的区域。最大位移、拉伸、压缩和剪切区域不重叠。位移和应变不是正态分布的。对侧眼睛的 IOP 反应并不总是比无关眼睛更相似。在高 IOP 下，一些 LC 区域处于双轴拉伸状态，其他区域处于双轴压缩状态。
我们在未经切割和固定的人类眼睛中以前所未有的分辨率获得了眼特异性的 IOP 对 LC 复杂影响的测量结果。我们的技术对电子和散斑噪声具有很强的鲁棒性。IOP 升高会导致 LC 产生显著的平面内拉伸和压缩。进一步的研究将在三维框架内探索 IOP 对 LC 的影响。
