Jaber Samer M, Hankenson F Claire, Heng Kathleen, McKinstry-Wu Andrew, Kelz Max B, Marx James O
University Laboratory Animal Resources, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
School of Veterinary Medicine, University of California, Davis, California, USA.
J Am Assoc Lab Anim Sci. 2014 Nov;53(6):684-91.
Extending a surgical plane of anesthesia in mice by using injectable anesthetics typically is accomplished by repeat-bolus dosing. We compared the safety and efficacy of redosing protocols administered either during an anesthetic surgical plane (maintaining a continuous surgical plane, CSP), or immediately after leaving this plane (interrupted surgical plane, ISP) in C57BL/6J mice. Anesthesia was induced with ketamine, xylazine, and acepromazine (80, 8, and 1 mg/kg IP, respectively), and redosing protocols included 25% (0.25K), 50% (0.5K), or 100% (1.0K) of the initial ketamine dose or 25% (0.25KX) or 50% (0.5KX) of the initial ketamine-xylazine dose. In the ISP group, the surgical plane was extended by 13.8 ± 2.1 min (mean ± SEM) after redosing for the 0.25K redose with 50% returning to a surgical plane, 42.7 ± 4.5 min for the 0.5K redose with 88% returning to a surgical plane, and 44.3 ± 15.4 min for the 1.0K redose, 52.8 ± 7.2 min for the 0.25KX redose, and 45.9 ± 2.9 min for the 0.5KX redose, with 100% of mice returning to a surgical plane of anesthesia in these 3 groups. Mortality rates for ISP groups were 0%, 12%, 33%, 12%, and 18%, respectively. Mice in CSP groups had 50% mortality, independent of the repeat-dosing protocol. We recommend redosing mice with either 50% of the initial ketamine dose or 25% of the initial ketamine-xylazine dose immediately upon return of the pedal withdrawal reflex to extend the surgical plane of anesthesia in mice, optimize the extension of the surgical plane, and minimize mortality.
通过使用可注射麻醉剂来延长小鼠的手术麻醉平面通常是通过重复推注给药来实现的。我们比较了在麻醉手术平面期间（维持连续手术平面，CSP）或离开该平面后立即（间断手术平面，ISP）给予再给药方案在C57BL/6J小鼠中的安全性和有效性。用氯胺酮、赛拉嗪和乙酰丙嗪（分别为80、8和1mg/kg腹腔注射）诱导麻醉，再给药方案包括初始氯胺酮剂量的25%（0.25K）、50%（0.5K）或100%（1.0K），或初始氯胺酮-赛拉嗪剂量的25%（0.25KX）或50%（0.5KX）。在ISP组中，再给予0.25K剂量后，手术平面延长了13.8±2.1分钟（平均值±标准误），50%的小鼠恢复到手术平面；给予0.5K剂量后为42.7±4.5分钟，88%的小鼠恢复到手术平面；给予1.0K剂量后为44.3±15.4分钟，给予0.25KX剂量后为52.8±7.2分钟，给予0.5KX剂量后为45.9±2.9分钟，这3组中100%的小鼠恢复到手术麻醉平面。ISP组的死亡率分别为0%、12%、33%、12%和18%。CSP组的小鼠死亡率为50%，与重复给药方案无关。我们建议在足趾退缩反射恢复后立即用初始氯胺酮剂量的50%或初始氯胺酮-赛拉嗪剂量的25%对小鼠进行再给药，以延长小鼠的手术麻醉平面，优化手术平面的延长，并将死亡率降至最低。
