Pawlowski Przemyslaw, Wawrusiewicz-Kurylonek Natalia, Eckstein Anja, Reszec Joanna, Luczynski Wlodzimierz, Johnson Kristian, Kretowski Adam, Bakunowicz-Lazarczyk Alina, Gorska Maria, Szamatowicz Jacek, Chyczewski Lech, Mysliwiec Janusz
Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, Poland ; Department of Pediatric Ophthalmology with Strabismus Treatment Unit, Medical University of Białystok, 17 Waszyngtona Street, 15-274 Białystok, Poland.
Department of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, Poland.
Mediators Inflamm. 2015;2015:340934. doi: 10.1155/2015/340934. Epub 2015 Jan 12.
Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves' orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n = 6) or severe GO (n = 20), and 7 healthy controls. The expression of mRNA of FOXP3, CTLA-4/CD28/CD80/CD86, CD40/CD40L was measured by RT-PCR. TCR and CD3 were evaluated by immunohistochemistry. Results. Higher mRNA for FoxP3 (relative expression: 1.4) and CD40 (1.27) and lower expression of CTLA-4 (0.61) were found in the GO tissues versus controls. In severe GO as compared to mild GO higher mRNA expression for FoxP3 (1.35) and CD40 (1.4) and lower expression for CTLA-4 (0.78), CD28 (0.62), and CD40L (0.56) were found. A positive correlation was found between FOXP3 mRNA and CD3 infiltration (R = 0.796, P = 0.0000001). Conclusions. The enhanced FOXP3 mRNA expression in GO samples may suggest the dysfunction of FOXP3 cells in the severe GO. The diminished mRNA expression of CTLA-4 in severe GO may indicate inadequate T regulatory function. The enhanced mRNA expression of CD40 in severe GO and negative correlation to CRP mRNA may suggest their role in the active and inactive GO.
目的。评估FOXP3、CTLA-4/CD28/CD80/CD86以及CD40/CD40L眼眶组织mRNA表达与Graves眼病（GO）严重程度之间的关系。材料与方法。从26例GO患者（轻度，n = 6；重度，n = 20）以及7例健康对照者获取眼眶组织。通过逆转录聚合酶链反应（RT-PCR）检测FOXP3、CTLA-4/CD28/CD80/CD86、CD40/CD40L的mRNA表达。通过免疫组织化学评估T细胞受体（TCR）和CD3。结果。与对照组相比，GO组织中FoxP3（相对表达：1.4）和CD40（1.27）的mRNA水平较高，而CTLA-4（0.61）的表达较低。与轻度GO相比，重度GO中FoxP3（1.35）和CD40（1.4）的mRNA表达较高，而CTLA-4（0.78）、CD28（0.62）和CD40L（0.56）的表达较低。发现FOXP3 mRNA与CD3浸润之间存在正相关（R = 0.796，P = 0.0000001）。结论。GO样本中FOXP3 mRNA表达增强可能提示重度GO中FOXP3细胞功能障碍。重度GO中CTLA-4 mRNA表达降低可能表明T调节功能不足。重度GO中CD40 mRNA表达增强以及与CRP mRNA的负相关可能提示它们在活动期和非活动期GO中的作用。
