Zobeiri Fatemeh, Sadrkhanlou Rajab-Ali, Salami Siamak, Mardani Karim, Ahmadi Abbas
Department of Basic Sciences, Histology and Embryology Section, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Clinical Biochemistry and Nutrition, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran;
Vet Res Forum. 2012 Spring;3(2):131-5.
Side effects of ciprofloxacin (CPFX), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. In the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm DNA damage using Aniline Blue and Acridine Orange technique, respectively. The fertility potential in male mice was also evaluated. NMRI male mice of 8-week old were included in this study and they were randomly divided into three groups. The first group was received low dose (LD) of ciprofloxacin (206 mg kg(-1), PO) and the second was treated with high dose (HD) of ciprofloxacin (412 mg kg(-1), PO) for 45 consecutive days. The control mice were only treated with oral carboxymethyl cellulose for 45 consecutive days. Sperm cells were removed from cauda epididymis and analyzed for chromatin integrity and DNA damage. In addition, the rate of fertilization, two cell embryos, blastocysts, arrested embryos and their types was examined using zygotes cultured in human tubal fluid - bovine serum albumin (HTF-BSA) medium. Concomitant significant increase in DNA damage and protamine deficiency of the sperm cells in ciprofloxacin treated mice were observed (P < 0.05). In addition, the fertilization rate and embryonic development in treated mice were significantly lower than that of control mice, but the embryo arrest rate in treated mice was significantly higher than that of control group (P < 0.001). In conclusion CPFX was able to induce DNA damage and chromatin abnormalities of sperm cells which could be contributed in the observed low fertilization rate and retarded embryonic development.
环丙沙星（CPFX）是一种广泛使用的、以氟喹诺酮为核心的广谱抗生素，已报道其在不同器官会产生副作用。在本研究中，我们分别使用苯胺蓝和吖啶橙技术，试图阐明环丙沙星对精子染色质完整性和精子DNA损伤的影响。还评估了雄性小鼠的生育潜力。本研究纳入了8周龄的NMRI雄性小鼠，并将它们随机分为三组。第一组接受低剂量（LD）的环丙沙星（206 mg kg⁻¹，口服），第二组连续45天接受高剂量（HD）的环丙沙星（412 mg kg⁻¹，口服）。对照小鼠仅连续45天口服羧甲基纤维素。从附睾尾部取出精子细胞，分析其染色质完整性和DNA损伤。此外，使用在人输卵管液-牛血清白蛋白（HTF-BSA）培养基中培养的受精卵，检查受精率、二细胞胚胎、囊胚、停滞胚胎及其类型。观察到环丙沙星处理的小鼠精子细胞的DNA损伤和鱼精蛋白缺乏同时显著增加（P < 0.05）。此外，处理组小鼠的受精率和胚胎发育明显低于对照组小鼠，但处理组小鼠的胚胎停滞率明显高于对照组（P < 0.001）。总之，环丙沙星能够诱导精子细胞的DNA损伤和染色质异常，这可能是观察到的低受精率和胚胎发育迟缓的原因。
