AlSaid Mansour, Mothana Ramzi, Raish Mohammad, Al-Sohaibani Mohammed, Al-Yahya Mohammed, Ahmad Ajaz, Al-Dosari Mohammed, Rafatullah Syed
Department of Pharmacognosy and Medicinal, Aromatic & Poisonous Plants Research Center (MAPPRC), College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Biomed Res Int. 2015;2015:359358. doi: 10.1155/2015/359358. Epub 2015 Jan 14.
Background. Liver diseases still represent a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Thus, the present study was to evaluate the effectiveness of Piper cubeba fruits in the amelioration of CCl4-induced liver injuries and oxidative damage in the rodent model. Methods. Hepatoprotective activity was assessed using various biochemical parameters like SGOT, SGPT, γ-GGT, ALP, total bilirubin, LDH, and total protein. Meanwhile, in vivo antioxidant activities as LPO, NP-SH, and CAT were measured in rat liver as well as mRNA expression of cytokines such as TNFα, IL-6, and IL-10 and stress related genes iNOS and HO-1 were determined by RT-PCR. The extent of liver damage was also analyzed through histopathological observations. Results. Treatment with PCEE significantly and dose dependently prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, PCEE significantly reduced the lipid peroxidation in the liver tissue and restored activities of defense antioxidant enzymes NP-SH and CAT towards normal levels. The administration of PCEE significantly downregulated the CCl4-induced proinflammatory cytokines TNFα and IL-6 mRNA expression in dose dependent manner, while it upregulated the IL-10 and induced hepatoprotective effect by downregulating mRNA expression of iNOS and HO-1 gene.
背景。肝脏疾病在全球范围内仍然是一个主要的健康负担。此外，药用植物在包括肝脏疾病在内的多种疾病治疗中越来越受欢迎。因此，本研究旨在评估荜澄茄果实对改善四氯化碳诱导的啮齿动物模型肝损伤和氧化损伤的有效性。方法。使用各种生化参数如谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）、γ-谷氨酰转肽酶（γ-GGT）、碱性磷酸酶（ALP）、总胆红素、乳酸脱氢酶（LDH）和总蛋白来评估肝脏保护活性。同时，测定大鼠肝脏中的脂质过氧化（LPO）、非蛋白巯基（NP-SH）和过氧化氢酶（CAT）等体内抗氧化活性，并通过逆转录聚合酶链反应（RT-PCR）测定细胞因子如肿瘤坏死因子α（TNFα）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）的mRNA表达以及应激相关基因诱导型一氧化氮合酶（iNOS）和血红素加氧酶-1（HO-1）。还通过组织病理学观察分析肝脏损伤程度。结果。荜澄茄乙醇提取物（PCEE）治疗显著且剂量依赖性地预防了药物诱导的血清肝酶水平升高。此外，PCEE显著降低了肝组织中的脂质过氧化，并将防御抗氧化酶NP-SH和CAT的活性恢复到正常水平。PCEE给药以剂量依赖性方式显著下调四氯化碳诱导的促炎细胞因子TNFα和IL-6的mRNA表达，同时上调IL-10，并通过下调iNOS和HO-1基因的mRNA表达诱导肝脏保护作用。
