Al-Said Mansour S, Mothana Ramzi A, Al-Yahya Mohammed M, Rafatullah Syed, Al-Sohaibani Mohammed O, Khaled Jamal M, Alatar Abdulrahman, Alharbi Naiyf S, Kurkcuoglu Mine, Baser Husnu C
Department of Pharmacognosy, and Medicinal, Aromatic & Poisonous Plants Research Center (MAPPRC), College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Department of Pathology, King Khalid University Hospital, King Saud University, P.O. Box 2925, Riyadh 11451, Saudi Arabia.
Evid Based Complement Alternat Med. 2016;2016:1867048. doi: 10.1155/2016/1867048. Epub 2016 May 12.
Liver disease is a worldwide problem. It represents one of the main causes of morbidity and mortality in humans. Achillea biebersteinii is used as herbal remedy for various ailments including liver diseases. But the scientific basis for its medicinal use remains unknown. Thus, this research was undertaken to evaluate the efficiency of A. biebersteinii essential oil (ABEO) (0.2 mL/kg) in the amelioration of CCl4-induced hepatotoxicity in rodent model. Moreover, the chemical content of the oil was investigated using GC and GC-MS. The following biochemical parameters were evaluated: serum glutamic oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), gamma-glutamyl-transpeptidase (γ-GGT), alkaline phosphatase (ALP), and total bilirubin. Furthermore, lipid profile, malondialdehyde (MDA), nonprotein sulfhydryl (NP-SH), and total protein (TP) contents in liver tissue were estimated. 44 components (92.0%) of the total oil have been identified by GC-MS analysis where α-terpinene and p-cymene were the most abundant. The high serum enzymatic (GOT, GPT, GGT, and ALP) and bilirubin concentrations as well as the level of MDA, NP-SH, and TP contents in liver tissues were significantly reinstated towards normalization by the ABEO. Histopathological study further confirmed these findings. In addition, ABEO showed mild antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid assays.
肝脏疾病是一个全球性问题。它是人类发病和死亡的主要原因之一。新疆一枝蒿被用作治疗包括肝脏疾病在内的各种疾病的草药。但其药用的科学依据仍然未知。因此，本研究旨在评估新疆一枝蒿精油（ABEO）（0.2 mL/kg）对啮齿动物模型中四氯化碳诱导的肝毒性的改善效果。此外，使用气相色谱（GC）和气相色谱-质谱联用（GC-MS）对该精油的化学成分进行了研究。评估了以下生化参数：血清谷草转氨酶（GOT）、谷丙转氨酶（GPT）、γ-谷氨酰转肽酶（γ-GGT）、碱性磷酸酶（ALP）和总胆红素。此外，还估计了肝组织中的脂质谱、丙二醛（MDA）、非蛋白巯基（NP-SH）和总蛋白（TP）含量。通过GC-MS分析鉴定出了该精油中44种成分（占总量的92.0%），其中α-萜品烯和对伞花烃含量最高。ABEO可使血清酶（GOT、GPT、GGT和ALP）和胆红素的高浓度以及肝组织中MDA、NP-SH和TP含量水平显著恢复至正常。组织病理学研究进一步证实了这些发现。此外，ABEO在2,2-二苯基-1-苦基肼基（DPPH）自由基清除和β-胡萝卜素-亚油酸测定中表现出轻度抗氧化活性。
