Efremova Yulia, Sinkorova Zuzana, Navratil Leos
1 Department of Health Care Disciplines and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague , Kladno, Czech Republic .
Photomed Laser Surg. 2015 Feb;33(2):82-91. doi: 10.1089/pho.2014.3824. Epub 2015 Feb 5.
The purpose of this study was to investigate the radioprotective features of 940 nm laser on the life span of mice, and absolute counts of blood cells and their proportions in gamma-irradiated mice.
An important feature of laser light is activation of mitotic division and differentiation of cells, which may be useful in activation of hematopoiesis in gamma-irradiated organisms.
Mice were randomly assigned to 11 groups according to the type(s) of influence. Generally, mice were irradiated in three different ways: with laser at different fluences, with gamma irradiation, or by combination of laser at different fluences and gamma irradiation in a different order. Mice were treated with 940 nm laser at 3, 12, or 18 J/cm(2) and/or a lethal dose of gamma irradiation (8.7 Gy). Each group was randomly subdivided into two subgroups, in which the life span of the mice and blood cell counts (on 12th and 45th day after gamma irradiation) were analyzed.
Laser (940 nm) at a fluence of 3 J/cm(2) significantly prolonged the life span of gamma-irradiated mice (p<0.05). In the same group, counts of white blood cells, lymphocytes, and neutrophils were higher on day 12 than in the gamma group. On day 45 after gamma irradiation, some signs of hematopoiesis repair were found in blood. There were no significant differences in counts of erythrocytes, monocytes, neutrophils, or the proportion of neutrophils between this group and the control group.
In summary, 940 nm laser at a fluence of 3 J/cm(2) demonstrates radioprotective features in an experiment with lethally irradiated mice. Mechanisms responsible for this effect will be investigated in further studies.
本研究旨在探讨940纳米激光对受γ射线照射小鼠寿命、血细胞绝对计数及其比例的辐射防护特性。
激光的一个重要特性是激活细胞的有丝分裂和分化，这可能有助于激活受γ射线照射生物体中的造血功能。
根据影响类型将小鼠随机分为11组。一般来说，小鼠以三种不同方式进行照射：不同能量密度的激光照射、γ射线照射，或不同能量密度的激光与γ射线以不同顺序联合照射。用能量密度为3、12或18 J/cm²的940纳米激光和/或致死剂量的γ射线（8.7 Gy）处理小鼠。每组随机再分为两个亚组，分析小鼠的寿命和血细胞计数（γ射线照射后第12天和第45天）。
能量密度为3 J/cm²的940纳米激光显著延长了受γ射线照射小鼠的寿命（p<0.05）。在同一组中，第12天时白细胞、淋巴细胞和中性粒细胞的计数高于γ射线照射组。γ射线照射后第45天，血液中发现了一些造血修复的迹象。该组与对照组在红细胞、单核细胞、中性粒细胞计数或中性粒细胞比例方面无显著差异。
总之，在对致死剂量照射小鼠的实验中，能量密度为3 J/cm²的940纳米激光表现出辐射防护特性。这种效应的作用机制将在进一步研究中进行探讨。
