Shields-Menard Sara A, Amirsadeghi Marta, Sukhbaatar Badamkhand, Revellame Emmanuel, Hernandez Rafael, Donaldson Janet R, French W Todd
Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA.
J Ind Microbiol Biotechnol. 2015 May;42(5):693-9. doi: 10.1007/s10295-014-1564-7. Epub 2015 Feb 6.
Biodiesel is an alternative fuel made from costly vegetable oil feedstocks. Some microorganisms can accumulate lipids when nutrients are limited and carbon is in excess. Rhodococcus rhodochrous is a gram-positive bacterium most often used in bioremediation or acrylamide production. The purpose of this study was to investigate and characterize the lipid accumulation capabilities of R. rhodochrous. Shake flasks and a large-scale fermentation were used to cultivate R. rhodochrous in varying concentrations of glucose. R. rhodochrous achieved almost 50 % of dry cell mass as lipid when grown in 20 g/L of glucose. Wax esters and triglycerides were identified in R. rhodochrous lipid extract. The transesterified extractables of R. rhodochrous consisted of mostly palmitic (35 %) and oleic (42 %) acid methyl esters. This study shows R. rhodochrous to be an oleaginous bacterium with potential for application in alternative fuels.
生物柴油是一种由昂贵的植物油原料制成的替代燃料。一些微生物在营养物质有限而碳过量时会积累脂质。红平红球菌是一种革兰氏阳性菌，最常用于生物修复或丙烯酰胺生产。本研究的目的是调查和表征红平红球菌的脂质积累能力。使用摇瓶和大规模发酵在不同浓度的葡萄糖中培养红平红球菌。当红平红球菌在20 g/L葡萄糖中生长时，其脂质含量几乎达到干细胞质量的50%。在红平红球菌的脂质提取物中鉴定出蜡酯和甘油三酯。红平红球菌的酯交换提取物主要由棕榈酸甲酯（35%）和油酸甲酯（42%）组成。本研究表明红平红球菌是一种产油细菌，具有应用于替代燃料的潜力。
