Wang Huili, Gao Ming, Wang Mei, Zhang Rongbo, Wang Wenwei, Dahlgren Randy A, Wang Xuedong
College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
College of Environmental Sciences and Public Health, Wenzhou Medical University, Wenzhou 325035, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Mar 15;985:62-70. doi: 10.1016/j.jchromb.2015.01.012. Epub 2015 Jan 22.
Herein, we developed a novel integrated device to perform phase separation based on ultrasound-assisted salt-induced liquid-liquid microextraction for determination of five fluoroquinones (FQs) in human body fluids. The integrated device consisted of three simple HDPE components used to separate the extraction solvent from the aqueous phase prior to retrieving the extractant. A series of extraction parameters were optimized using the response surface method based on central composite design. Optimal conditions consisted of 945μL acetone extraction solvent, pH 2.1, 4.1min stir time, 5.9g Na2SO4, and 4.0min centrifugation. Under optimized conditions, the limits of detection (at S/N=3) were 0.12-0.66μgL(-1), the linear range was 0.5-500μgL(-1) and recoveries were 92.6-110.9% for the five FQs extracted from plasma and urine. The proposed method has several advantages, such as easy construction from inexpensive materials, high extraction efficiency, short extraction time, and compatibility with HPLC analysis. Thus, this method shows excellent prospects for sample pretreatment and analysis of FQs in human body fluids.
在此，我们开发了一种新型集成装置，基于超声辅助盐诱导液-液微萃取进行相分离，用于测定人体体液中的五种氟喹诺酮类药物（FQs）。该集成装置由三个简单的高密度聚乙烯组件组成，用于在回收萃取剂之前将萃取溶剂与水相分离。基于中心复合设计，采用响应面法对一系列萃取参数进行了优化。最佳条件包括945μL丙酮萃取溶剂、pH值2.1、搅拌时间4.1分钟、5.9g硫酸钠以及离心时间4.0分钟。在优化条件下，从血浆和尿液中萃取的五种FQs的检测限（S/N = 3时）为0.12 - 0.66μgL(-1)，线性范围为0.5 - 500μgL(-1)，回收率为92.6 - 110.9%。所提出的方法具有几个优点，例如由廉价材料易于构建、萃取效率高、萃取时间短以及与高效液相色谱分析兼容。因此，该方法在人体体液中FQs的样品预处理和分析方面显示出极好的前景。
