Njumbe Ediage Emmanuel, Van Poucke Christof, De Saeger Sarah
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Gent, Belgium.
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Gent, Belgium.
Food Chem. 2015 Jun 15;177:397-404. doi: 10.1016/j.foodchem.2015.01.060. Epub 2015 Jan 17.
An LC-MS/MS method was developed and validated for the detection and quantification of 23 mycotoxins in different varieties of sorghum. The method performance characteristics were as follows: suitable linearity ranges for all 23 mycotoxins with p-value >0.05; limits of detection (1.2-50 μg/kg), limits of quantification (2.5-100 μg/kg), repeatability (RSDr, 7-22%), intermediate precision (RSDR, 14-44%) and apparent recovery (0.2-11%, expressed as bias). The method was applied to analyze 10 samples obtained from retail shops in Belgium (n=8) and Germany (n=2). Nine of the 10 samples (90%) were positive for the following mycotoxins: aflatoxin B1 (50 μg/kg), alternariol monomethyl ether (<LOQ - 79 μg/kg), alternariol (303-357 μg/kg), diacetoxyscirpenol (<LOQ - 91 μg/kg), fumonisin B1 (<LOQ - 95 μg/kg), fumonisin B2 (<LOQ), fumonisin B3 (<LOQ), T2 (<LOQ) and zearalenone (<LOQ).
建立了一种液相色谱-串联质谱（LC-MS/MS）方法，用于检测和定量不同品种高粱中的23种霉菌毒素。该方法的性能特征如下：所有23种霉菌毒素的线性范围合适，p值>0.05；检测限（1.2 - 50μg/kg）、定量限（2.5 - 100μg/kg）、重复性（RSDr，7 - 22%）、中间精密度（RSDR，14 - 44%）和表观回收率（0.2 - 11%，以偏差表示）。该方法应用于分析从比利时（n = 8）和德国（n = 2）零售商店获得的10个样品。10个样品中有9个（90%）对以下霉菌毒素呈阳性：黄曲霉毒素B1（50μg/kg）、单甲基交替菌素醚（<定量限 - 79μg/kg）、交替菌素（303 - 357μg/kg）、二乙酰基草镰刀菌烯醇（<定量限 - 91μg/kg）、伏马菌素B1（<定量限 - 95μg/kg）、伏马菌素B2（<定量限）、伏马菌素B3（<定量限）、T - 2毒素（<定量限）和玉米赤霉烯酮（<定量限）。
