Li Bo, Pratt Derek A
Department of Chemistry, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
Department of Chemistry, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
Free Radic Biol Med. 2015 May;82:187-202. doi: 10.1016/j.freeradbiomed.2015.01.020. Epub 2015 Feb 4.
Hydrocarbon autoxidation is the free radical chain reaction primarily responsible for the oxidative degradation of organic materials, including those that make up cells, tissues, and organs. The identification of compounds that slow this process (antioxidants) and the quantitation of their efficacies have long been goals of academic and industrial researchers. Antioxidants are generally divided into two types: preventive and radical-trapping (also commonly referred to as chain-breaking). Preventive antioxidants slow the rate of initiation of autoxidation, whereas radical-trapping antioxidants slow the rate of propagation by reacting with chain-propagating peroxyl radicals. The purpose of this review is to provide a comprehensive overview of different approaches to measure the kinetics of the reactions of radical-trapping antioxidants with peroxyl radicals, and their use to study the inhibition of hydrocarbon (lipid) autoxidation in homogeneous solution, as well as biphasic media (lipid bilayers) and cell culture. Direct and indirect approaches are presented and advantages and disadvantages of each are discussed in order to facilitate method selection for investigators seeking to address particular questions in this immensely popular field.
烃类自动氧化是一种自由基链式反应，主要导致有机材料的氧化降解，包括构成细胞、组织和器官的那些材料。识别减缓这一过程的化合物（抗氧化剂）并定量其功效，长期以来一直是学术和工业研究人员的目标。抗氧化剂通常分为两类：预防性抗氧化剂和自由基捕获型抗氧化剂（也通常称为链断裂型抗氧化剂）。预防性抗氧化剂减缓自动氧化的引发速率，而自由基捕获型抗氧化剂通过与链增长的过氧自由基反应来减缓传播速率。本综述的目的是全面概述测量自由基捕获型抗氧化剂与过氧自由基反应动力学的不同方法，以及它们在研究均相溶液、双相介质（脂质双层）和细胞培养中烃类（脂质）自动氧化抑制方面的应用。文中介绍了直接和间接方法，并讨论了每种方法的优缺点，以便为试图解决这个非常热门领域中特定问题的研究人员提供方法选择方面的便利。
