Yokota Shin-ichi, Konno Mutsuko, Fujiwara Shin-ichi, Toita Nariaki, Takahashi Michiko, Yamamoto Soh, Ogasawara Noriko, Shiraishi Tsukasa
Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Department of Pediatrics, Sapporo Kosei General Hospital, Sapporo, Japan.
Helicobacter. 2015 Oct;20(5):334-42. doi: 10.1111/hel.12217. Epub 2015 Feb 9.
The infection route of Helicobacter pylori has been recognized to be mainly intrafamilial, preferentially mother-to-child, especially in developed countries. To determine the transmission route, we examined whether multilocus sequence typing (MLST) was useful for analysis of intrafamilial infection. The possibility of intraspousal infection was also evaluated.
Clonal relationships between strains derived from 35 index Japanese pediatric patients, and their family members were analyzed by two genetic typing procedures, MLST and random amplified polymorphic DNA (RAPD) fingerprinting.
Mostly coincident results were obtained by MLST and RAPD. By MLST, the allele of loci in the isolates mostly matched between the index child and both the father and mother for 9 (25.7%) of the 35 patients, between the index child and the mother for 25 (60.0%) of the 35 patients.
MLST is useful for analyzing the infection route of H. pylori as a highly reproducible method. Intrafamilial, especially mother-to-children and sibling, infection is the dominant transmission route. Intraspousal infection is also thought to occur in about a quarter in the Japanese families.
幽门螺杆菌的感染途径已被认为主要是家庭内传播，尤其是母婴传播，在发达国家更是如此。为了确定传播途径，我们研究了多位点序列分型（MLST）是否有助于分析家庭内感染情况。同时也评估了配偶间感染的可能性。
通过两种基因分型方法，即MLST和随机扩增多态性DNA（RAPD）指纹图谱分析，对35例日本儿科患者及其家庭成员的菌株间克隆关系进行分析。
MLST和RAPD得到的结果大多一致。通过MLST分析，在35例患者中，有9例（25.7%）的患儿分离株与父母双方的位点等位基因大多匹配，有25例（60.0%）的患儿分离株与母亲的位点等位基因大多匹配。
MLST作为一种高度可重复的方法，有助于分析幽门螺杆菌的感染途径。家庭内感染，尤其是母婴和兄弟姐妹间感染是主要传播途径。在日本家庭中，配偶间感染也被认为约占四分之一。
