Hossain A, Bakir T F, Siddiqui M A, Khawaja M M, De-Silva S, Sengupta B S, el-Sheikh M M, Madani M E
Department of Pathology, College of Medicine, King Saud University, Saudi Arabia.
Int J Gynaecol Obstet. 1989 May;29(1):51-6. doi: 10.1016/0020-7292(89)90129-x.
The laboratory diagnosis of genital herpes simplex virus (HSV) infections in various patient groups with diverse clinical manifestations including asymptomatic pregnant women with history of genital herpes and in a high prevalence group consisting of male patients with recurring genital ulcerations is described. HSV was detected by conventional cell culture and also by a CPE-enhancement technique using human embryonic lung fibroblasts (MRC-5) and Vero cells with subsequent typing of isolate by HSV type-specific monoclonal antibodies. A definite improvement in HSV diagnosis was noted by the use of the CPE-enhancement technique with the reduction of turn-around time to 24 h at which 61.1% and 50% isolation rates were detected in MRC-5 cells in comparison to 38.9% and 16.6% by conventional cell culture in the high prevalence patient population and asymptomatic pregnant women, respectively. Delayed development of CPE and reduced isolation rates were detected using Vero cells. Follow-up sampling of subsequent episodes of genital ulcerations in those with history of recurrence but an initial negative cell culture result, increased the isolation rate substantially and enabled the confirmation of diagnosis of genital HSV infection.
本文描述了对不同患者群体生殖器单纯疱疹病毒（HSV）感染的实验室诊断，这些患者群体临床表现各异，包括有生殖器疱疹病史的无症状孕妇以及由复发性生殖器溃疡男性患者组成的高患病率群体。通过传统细胞培养以及使用人胚肺成纤维细胞（MRC - 5）和非洲绿猴肾细胞（Vero细胞）的细胞病变效应（CPE）增强技术检测HSV，并随后使用HSV型特异性单克隆抗体对分离株进行分型。使用CPE增强技术可显著改善HSV诊断，将周转时间缩短至24小时，在高患病率患者群体和无症状孕妇中，MRC - 5细胞的分离率分别为61.1%和50%，而传统细胞培养的分离率分别为38.9%和16.6%。使用Vero细胞检测到CPE出现延迟且分离率降低。对有复发史但初始细胞培养结果为阴性的患者，在随后生殖器溃疡发作时进行随访采样，可大幅提高分离率并确诊生殖器HSV感染。
