Volpi A, Lakeman A D, Pereira L, Stagno S
Am J Obstet Gynecol. 1983 Aug 1;146(7):813-5. doi: 10.1016/0002-9378(83)91083-9.
Fluorescein-conjugated, type-specific monoclonal antibodies to herpes simplex virus (HSV-1 and HSV-2) and group-specific antibody which recognizes both HSV-1 and HSV-2 were used to detect HSV-infected cells in clinical specimens. Specimens were collected from 66 pregnant women with a past history of herpes genitalis or with suspected lesions. Fourteen of 18 samples from which virus was isolated were positive by immunofluorescence test; four of 18 specimens had an insufficient number of cells (less than 50 per smear) for analysis. In one specimen, a positive reaction by immunofluorescence was not confirmed by virus isolation. HSV was typed directly on smears of clinical specimens in 14 instances: three samples were identified as HSV-1, and 11 as HSV-2. Moreover, the immunofluorescence test was used to type HSV isolates in cell cultures in these cases and 40 additional strains. Examination of viral deoxyribonucleic acid fragments obtained by restriction enzyme digestion confirmed the typings; complete agreement was found among the three methods. Immunofluorescence with monoclonal antibodies is a reliable test for rapid diagnosis and simultaneous typing of genital HSV infections, even in asymptomatic women. With adequate specimens, the specificity and sensitivity of this method approach 100%.
用与单纯疱疹病毒（HSV-1和HSV-2）结合的荧光素标记的型特异性单克隆抗体以及识别HSV-1和HSV-2的群特异性抗体来检测临床标本中的HSV感染细胞。标本取自66名有生殖器疱疹病史或有疑似病变的孕妇。在18份分离出病毒的样本中，14份通过免疫荧光试验呈阳性；18份标本中有4份细胞数量不足（每份涂片少于50个），无法进行分析。在一份标本中，免疫荧光阳性反应未得到病毒分离的证实。在14例临床标本涂片中直接对HSV进行分型：3份样本被鉴定为HSV-1，11份为HSV-2。此外，在这些病例以及另外40株毒株的细胞培养物中，用免疫荧光试验对HSV分离株进行分型。对通过限制性酶切获得的病毒脱氧核糖核酸片段的检测证实了分型结果；三种方法之间完全一致。用单克隆抗体进行免疫荧光是一种可靠的检测方法，可用于生殖器HSV感染的快速诊断和同时分型，即使是无症状女性。有足够的标本时，该方法的特异性和敏感性接近100%。
