Sugiyama K, Matsuura Y, Morita C, Shiga S, Akao Y, Komatsu T, Kitamura T
J Infect Dis. 1984 Jan;149(1):67-73. doi: 10.1093/infdis/149.1.67.
Antigens of the viruses that cause hemorrhagic fever with renal syndrome (HFRS) and antibodies to these antigens were titrated in an immune adherence hemagglutination (IAHA) test and a CF test. Vero-E6 cells infected with the SR-11 strain of HFRS virus, an isolate from rats associated with a laboratory outbreak in Japan, and the 76-118 strain of Hantaan virus, the etiologic agent of Korean hemorrhagic fever, were used as antigens in the IAHA, CF, and indirect immunofluorescent antibody (IFA) tests. Sera from patients with different types of HFRS were tested against both strains of virus. Titers of antibody detected by the IFA test were similar for the two strains, but the IAHA test discriminated between antibodies to the two types of HFRS virus. Antigenic differences between HFRS viruses were suggested by the results of the IAHA test.
在免疫粘连血凝试验（IAHA）和补体结合试验（CF）中，对引起肾综合征出血热（HFRS）的病毒抗原以及针对这些抗原的抗体进行了滴定。用感染了HFRS病毒SR - 11株（从日本一次实验室爆发相关大鼠中分离得到）和汉坦病毒76 - 118株（朝鲜出血热病原体）的非洲绿猴肾细胞（Vero - E6）作为IAHA、CF和间接免疫荧光抗体（IFA）试验的抗原。对不同类型HFRS患者的血清进行了两种病毒株的检测。IFA试验检测到的两种病毒株抗体滴度相似，但IAHA试验能区分针对两种类型HFRS病毒的抗体。IAHA试验结果提示了HFRS病毒之间的抗原差异。
