Barbul A, Shawe T, Rotter S M, Efron J E, Wasserkrug H L, Badawy S B
Department of Surgery, Sinai Hospital of Baltimore, Md.
Surgery. 1989 Jun;105(6):764-9.
In order to understand the role of T cells in postinjury fibroplasia, we have studied wound healing in congenitally athymic nude mice that lack a normally developed T cell system. Healing of incisional wounds, as assessed by wound breaking strength, was significantly stronger in nude mice compared with normal thymus-bearing animals. This was accompanied by a marked increase in the amount of reparative collagen synthesized at the wound site, as assessed by the hydroxyproline content of subcutaneously implanted sponges. Because nude mice have some extrathymic T cell maturation, we used an anti-Thy-1.2 (30H12) monoclonal antibody to selectively deplete T cells in vivo. Although such treatments impaired wound healing in normal mice, they had no effect on any wound healing parameter in nude mice. In a separate experiment, T cell reconstitution of nude mice, sufficient to significantly enhance in vivo delayed hypersensitivity responses, led to a decrease in both wound breaking strength and hydroxyproline deposition in subcutaneously implanted polyvinyl sponges. The data suggest that T cells play a dual role in wound healing: an early stimulatory role on macrophages, endothelial cells, and fibroblasts, and a late counterregulatory role, which may be responsible for the orderly completion of wound repair.
为了了解T细胞在损伤后纤维组织形成中的作用，我们研究了先天性无胸腺裸鼠（缺乏正常发育的T细胞系统）的伤口愈合情况。通过伤口抗张强度评估，裸鼠切口伤口的愈合情况明显强于正常有胸腺动物。皮下植入海绵的羟脯氨酸含量评估显示，这伴随着伤口部位合成的修复性胶原蛋白量显著增加。由于裸鼠有一些胸腺外T细胞成熟，我们使用抗Thy-1.2（30H12）单克隆抗体在体内选择性清除T细胞。虽然这种处理损害了正常小鼠的伤口愈合，但对裸鼠的任何伤口愈合参数都没有影响。在另一个实验中，对裸鼠进行T细胞重建，足以显著增强体内迟发型超敏反应，导致皮下植入的聚乙烯海绵的伤口抗张强度和羟脯氨酸沉积均降低。数据表明，T细胞在伤口愈合中起双重作用：早期对巨噬细胞、内皮细胞和成纤维细胞有刺激作用，后期起反调节作用，这可能负责伤口修复的有序完成。
