酶联免疫吸附测定法与直接显微镜检查法在粪便样本中诊断肠道贾第虫的比较分析
Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples.
作者信息
Singhal Shipra, Mittal Vineeta, Khare Vineeta, Singh Yengkokpam Ibotomba
机构信息
Department of Microbiology, Era's Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India.
出版信息
Indian J Pathol Microbiol. 2015 Jan-Mar;58(1):69-71. doi: 10.4103/0377-4929.151192.
CONTEXT
Giardiasis is one of the most common nonviral infections causing diarrheal illness worldwide. In this prospective cross-sectional study, we evaluated the RIDASCREEN ® Giardia kit for detection of Giardia intestinalis in stool samples and compared the results with direct microscopy.
MATERIALS AND METHODS
A total of 360 fecal samples were collected. They were then processed by wet film, iodine preparation and an enzyme-linked immunosorbent assay (ELISA) kit to determine the presence of Giardia trophozoites and cysts. Statistical analysis was performed by sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy.
RESULTS AND CONCLUSION
Of the 360 cases, 17.2% samples were positive for Giardia by direct microscopy and 23.6% were found to be positive by ELISA (sensitivity ~97%), but specificity was ~92% only. Because of less specificity, we need to perform ELISA in congruence with direct microscopy, etc. Further studies need to be performed on a larger sample size using other molecular tests in order to get more accurate estimations.
背景
贾第虫病是全球范围内引起腹泻疾病的最常见非病毒感染之一。在这项前瞻性横断面研究中，我们评估了RIDASCREEN®贾第虫检测试剂盒用于检测粪便样本中肠贾第虫的情况，并将结果与直接显微镜检查进行比较。
材料与方法
共收集了360份粪便样本。然后通过湿片法、碘染色法和酶联免疫吸附测定（ELISA）试剂盒对样本进行处理，以确定贾第虫滋养体和包囊的存在情况。通过敏感性、特异性、阳性预测值、阴性预测值和诊断准确性进行统计分析。
结果与结论
在360例病例中，直接显微镜检查显示17.2%的样本贾第虫呈阳性，ELISA检测发现23.6%呈阳性（敏感性约为97%），但特异性仅约为92%。由于特异性较低，我们需要将ELISA与直接显微镜检查等方法结合使用。需要使用其他分子检测方法对更大样本量进行进一步研究，以便获得更准确的估计。
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