Yagci Filiz, Kesim Bulent
Department of Prosthodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey.
Gerodontology. 2016 Sep;33(3):421-7. doi: 10.1111/ger.12191. Epub 2015 Feb 12.
To investigate cytotoxic and genotoxic effects of static magnetic field (SMF) produced by dental magnetic attachments on human gingival fibroblasts in vitro.
Magnetic attachments have numerous roles in dental prosthesis fixation, but few reports evaluate possible biological effects of static magnetic field (SMF) on human gingival tissues, particular genotoxic effects.
The Dyna (500-gr breakaway force) and Steco (173-gr breakaway force) dental magnetic attachments were embedded into autopolymerising acrylic resin in four different configurations each, including single and double magnets. Gingival biopsy was performed on 28 individuals during third molar extraction, and each sample was divided into two pieces for culture under SMF exposure or as a control. In total, seven test and seven control gingival fibroblast cultures were performed for each group resulting in 56 gingival fibroblast cultures. The test culture flasks were placed atop the magnet-embedded resin blocks. After cultures were terminated, mitotic index (MI) and micronucleus (MN) rates were analysed at a p = 0.05 significance level by Wilcoxon's test; intergroup differences were analysed with a Kruskal-Wallis test.
There was no significant difference in intragroup or intergroup MI rates. The double Dyna (p = 0.023) and double Steco (p = 0.016) groups had statistically significant intragroup differences in the MN rates. There were no statistically significant differences in MN rates in intergroup analyses.
In particular, higher magnetic fields from dental magnetic attachments might be toxic genetically to human gingival fibroblasts. However, there is need for further investigations from different aspects to detect any genotoxicity.
研究牙科磁性附件产生的静磁场（SMF）对体外培养的人牙龈成纤维细胞的细胞毒性和遗传毒性作用。
磁性附件在假牙固定中具有多种作用，但很少有报告评估静磁场（SMF）对人牙龈组织可能产生的生物学效应，尤其是遗传毒性作用。
将Dyna（500克分离力）和Steco（173克分离力）牙科磁性附件以四种不同配置分别嵌入自凝丙烯酸树脂中，包括单磁体和双磁体。在28名个体拔除第三磨牙时进行牙龈活检，每个样本分成两块，分别在静磁场暴露下培养或作为对照。每组共进行7次测试和7次对照牙龈成纤维细胞培养，共得到56个牙龈成纤维细胞培养物。测试培养瓶放置在嵌入磁体的树脂块上。培养结束后，通过Wilcoxon检验在p = 0.05显著性水平下分析有丝分裂指数（MI）和微核（MN）率；组间差异用Kruskal-Wallis检验分析。
组内或组间MI率无显著差异。双Dyna组（p = 0.023）和双Steco组（p = 0.016）的MN率在组内有统计学显著差异。组间分析中MN率无统计学显著差异。
特别是，牙科磁性附件产生的较高磁场可能对人牙龈成纤维细胞具有遗传毒性。然而，需要从不同方面进行进一步研究以检测任何遗传毒性。
