利用荧光激活细胞分选技术建立细胞系的新方法，产生了6种新的头颈部鳞状细胞癌系。
Novel method of cell line establishment utilizing fluorescence-activated cell sorting resulting in 6 new head and neck squamous cell carcinoma lines.
作者信息
Owen John Henry, Graham Martin P, Chinn Steven B, Darr Owen F, Chepeha Douglas B, Wolf Gregory T, Bradford Carol R, Carey Thomas E, Prince Mark E P
机构信息
Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
出版信息
Head Neck. 2016 Apr;38 Suppl 1(Suppl 1):E459-67. doi: 10.1002/hed.24019. Epub 2015 Jul 14.
BACKGROUND
The purpose of this study was to present the establishment of new cell lines, which is important to cancer research.
METHODS
Six new head and neck squamous cell carcinoma cell lines were established using a novel fluorescence-activated cell sorting (FACS) method in order to overcome the barrier of fibroblast overgrowth and the susceptibility of primary tumors to fail in vitro.
RESULTS
Antibodies chosen for specific targeting of epithelial cells and fibroblasts successfully separated cells for line establishment in 6 of 12 attempts, providing an alternative method of establishing head and neck squamous cell carcinoma cell lines. Each attempt at cell line establishment resulted in an epithelial carcinoma population, which was genotyped and catalogued as a unique cell line, and a corresponding fibroblast population.
CONCLUSION
The selection of antibody markers could be optimized to aid in the establishment of any cancer cell line derived from any tumor tissue; this method is not limited to head and neck cancer. © 2015 Wiley Periodicals, Inc. Head Neck 38: E459-E467, 2016.
背景
本研究的目的是介绍新细胞系的建立，这对癌症研究很重要。
方法
为克服成纤维细胞过度生长的障碍以及原发性肿瘤在体外培养失败的易感性，采用一种新型荧光激活细胞分选（FACS）方法建立了六种新的头颈部鳞状细胞癌细胞系。
结果
用于特异性靶向上皮细胞和成纤维细胞的抗体在12次尝试中的6次成功分离出用于细胞系建立的细胞，提供了一种建立头颈部鳞状细胞癌细胞系的替代方法。每次细胞系建立尝试都产生了一个上皮癌细胞群体，对其进行基因分型并作为一个独特的细胞系进行编目，以及一个相应的成纤维细胞群体。
结论
可以优化抗体标志物的选择，以帮助建立源自任何肿瘤组织的任何癌细胞系；这种方法不限于头颈癌。©2015威利期刊公司。《头颈》38: E459 - E467，2016。
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