University of Granada, Faculty of Dentistry, Dental Materials Section, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
University of Granada, NanoMyP. Spin-Off Enterprise, Edificio BIC-Granada, Av. Innovación 1, 18016 - Armilla, Granada, Spain.
Acta Biomater. 2018 May;72:371-380. doi: 10.1016/j.actbio.2018.03.033. Epub 2018 Mar 24.
The aim of this study was to evaluate the effectiveness of different nanoparticles-based solutions for dentin permeability reduction and to determine the viscoelastic performance of cervical dentin after their application. Four experimental nanoparticle solutions based on zinc, calcium or doxycycline-loaded polymeric nanoparticles (NPs) were applied on citric acid etched dentin, to facilitate the occlusion and the reduction of the fluid flow at the dentinal tubules. After 24 h and 7 d of storage, cervical dentin was evaluated for fluid filtration. Field emission scanning electron microscopy, energy dispersive analysis, AFM and Nano-DMA analysis were also performed. Complex, storage, loss modulus and tan delta (δ) were assessed. Doxycycline-loaded NPs impaired tubule occlusion and fluid flow reduction trough dentin. Tubules were 100% occluded in dentin treated with calcium-loaded NPs or zinc-loaded NPs, analyzed at 7 d. Dentin treated with both zinc-NPs and calcium-NPs attained the highest reduction of dentinal fluid flow. Moreover, when treating dentin with zinc-NPs, complex modulus values attained at intertubular and peritubular dentin were higher than those obtained after applying calcium-NPs. Zinc-NPs are then supposed to fasten active dentin remodeling, with increased maturity and high mechanical properties. Zinc-based nanoparticles are then proposed for effective dentin remineralization and tubular occlusion. Further research to finally prove for clinical benefits in patients with dentin hypersensitivity using Zn-doped nanoparticles is encouraged.
Erosion from acids provokes dentin hypersensitivity (DH) which presents with intense pain of short duration. Open dentinal tubules and demineralization favor DH. Nanogels based on Ca-nanoparticles and Zn-nanoparticles produced an efficient reduction of fluid flow. Dentinal tubules were filled by precipitation of induced calcium-phosphate deposits. When treating dentin with Zn-nanoparticles, complex modulus values attained at intertubular and peritubular dentin were higher than those obtained after applying Ca-nanoparticles. Zn-nanoparticles are then supposed to fasten active dentin remodeling, with increased maturity and high mechanical properties. Zinc-based nanogels are, therefore, proposed for effective dentin remineralization and tubular occlusion. Further research to finally prove for clinical benefits in patients with dentin hypersensitivity using Zn-doped nanogels is encouraged.
本研究旨在评估不同基于纳米粒子的溶液对牙本质渗透性降低的效果，并确定应用这些溶液后牙本质颈部的黏弹性能。四种基于锌、钙或载多西环素聚合物纳米颗粒（NPs）的实验纳米粒子溶液被应用于柠檬酸蚀刻牙本质，以促进牙本质小管的封闭和减少流体流动。在储存 24 小时和 7 天后，评估牙本质颈部的流体过滤情况。还进行了场发射扫描电子显微镜、能量色散分析、原子力显微镜和纳米 DMA 分析。评估了复数模量、储能模量、损耗模量和损耗角正切（δ）。载多西环素的 NPs 通过牙本质阻碍小管封闭和减少流体流动。用载钙 NPs 或载锌 NPs 处理的牙本质中，小管 100%被封闭，在 7 天分析时。用锌 NPs 和钙 NPs 处理的牙本质均实现了牙本质流体流动的最大减少。此外，当用锌 NPs 处理牙本质时，在管间和管周牙本质中获得的复数模量值高于用钙 NPs 处理后的获得的复数模量值。因此，锌 NPs 被认为可以加速活跃的牙本质重塑，提高成熟度和高机械性能。因此，锌基纳米粒子被提出用于有效牙本质再矿化和小管封闭。鼓励进一步研究，最终证明使用掺锌纳米粒子对牙本质过敏症患者的临床益处。
