Ogasawara T, Ohnhaus E E, Hoensch H P
Department of Internal Medicine, University of Essen, Federal Republic of Germany.
Res Exp Med (Berl). 1989;189(3):195-204. doi: 10.1007/BF01852168.
Starvation for 24 h causes a striking fall in glutathione content from 3.19 +/- 0.27 to 1.88 +/- 0.14 (X +/- SEM) mumol/g tissue and of GGT activity from 31.75 +/- 4.17 to 19.49 +/- 3.13 (X +/- SEM) nmol/min/mg protein in the homogenate from whole mucosa of the upper small intestinal segments. This was associated with a significant increase in GSH-Px activity and the content of lipid peroxides (measured by the thiobarbituric assay). On semi-synthetic iron-supplemented diet the activities of GSH-T and GGT were significantly decreased as compared with crude diet. On semisynthetic iron-depleted diet GSH-T and GGT activities were further depressed, but this was accompanied with an additional depression of GSH, glutathione reductase (GSSG-R), and glutathione peroxidase (GSH-Px) activities and lipid peroxide concentrations. Food deprivation significantly lowers the mucosal GSH-content and could lead to a destabilization of this system presumably by increased oxidative stress. As compared to normal "crude" diet, semisynthetic diets and oral iron depletion have been shown to cause a depression of the intestinal GSH system. As a consequence of these effects, the resistance of the small intestinal mucosa toward exogeneous dietary toxins might be reduced.
饥饿24小时会导致小肠上段全层黏膜匀浆中谷胱甘肽含量显著下降，从3.19±0.27降至1.88±0.14（X±SEM）μmol/g组织，γ-谷氨酰转肽酶（GGT）活性从31.75±4.17降至19.49±3.13（X±SEM）nmol/min/mg蛋白质。这与谷胱甘肽过氧化物酶（GSH-Px）活性及脂质过氧化物含量（通过硫代巴比妥酸法测定）的显著增加相关。与天然饲料相比，半合成补铁饲料组中谷胱甘肽转移酶（GSH-T）和GGT的活性显著降低。在半合成缺铁饲料组中，GSH-T和GGT的活性进一步降低，但同时谷胱甘肽（GSH）、谷胱甘肽还原酶（GSSG-R）和谷胱甘肽过氧化物酶（GSH-Px）的活性以及脂质过氧化物浓度也进一步降低。食物缺乏显著降低了黏膜中GSH的含量，可能由于氧化应激增加导致该系统不稳定。与正常“天然”饲料相比，半合成饲料和口服缺铁已被证明会导致肠道GSH系统功能降低。这些影响的结果可能是小肠黏膜对外源性饮食毒素的抵抗力降低。
