Department of Materials Science & Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan; Department of Mechanical Engineering, Manad State University (UNIMA), Indonesia.
Department of Materials Science & Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:430-435. doi: 10.1016/j.msec.2015.01.031. Epub 2015 Jan 8.
The surface wettability of implants is a crucial factor in their osteoconductivity because it influences the adsorption of cell-attached proteins onto the surface. In this study, a single-step hydrothermal surface treatment using distilled water at a temperature of 180°C for 3h was applied to titanium (Ti) and its alloys (Ti-6Al-4V, Ti-6Al-7Nb, Ti-29Nb-13Ta-4.6Zr, Ti-13Cr-1Fe-3Al; mass%) and compared with as-polished Ti implants and with implants produced by anodizing Ti in 0.1M of H3PO4 with applied voltages from 0V to 150V at a scanning rate of 0.1Vs(-1). The surface-treated samples were stored in a five time phosphate buffered saline (×5 PBS(-)) solution to prevent increasing the water contact angle (WCA) with time. The surface characteristics were evaluated using scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Auger electron spectroscopy, surface roughness, and contact angle measurement using a 2μL droplet of distilled water. The relationship between WCA and osteoconductivity at various surface modifications was examined using in vivo tests. The results showed that a superhydrophilic surface with a WCA≤10° and a high osteoconductivity (RB-I) of up to 50% in the cortical bone part, about four times higher than the as-polished Ti and Ti alloys, were provided by the combination of the hydrothermal surface treatment and storage in ×5 of PBS(-).
植入物的表面润湿性是其骨传导性的一个关键因素，因为它会影响细胞附着蛋白吸附到表面上。在这项研究中，采用 180°C 去离子水单步水热处理 3h 应用于钛（Ti）及其合金（Ti-6Al-4V、Ti-6Al-7Nb、Ti-29Nb-13Ta-4.6Zr、Ti-13Cr-1Fe-3Al；质量%），并与抛光 Ti 植入物和在 0.1M 的 H3PO4 中通过施加 0V 至 150V 电压在 0.1Vs(-1) 的扫描速率阳极氧化 Ti 制成的植入物进行比较。将表面处理的样品储存在五倍磷酸盐缓冲盐水（×5PBS(-)）溶液中，以防止随时间推移增加水接触角（WCA）。使用扫描电子显微镜、X 射线衍射、X 射线光电子能谱、俄歇电子能谱、表面粗糙度和使用 2μL 去离子水滴的接触角测量来评估表面特性。使用体内测试检查各种表面改性的 WCA 与骨传导性之间的关系。结果表明，水热处理和在×5PBS(-)中储存的组合提供了具有 WCA≤10°的超亲水表面和高达 50%的皮质骨部分的高骨传导性（RB-I），比抛光 Ti 和 Ti 合金高约 4 倍。
