Lazić Gospava, Grubač Siniša, Lupulović Diana, Bugarski Dejan, Lazić Sava, Knežević Petar, Petrović Tamaš
Scientific Veterinary Institute "Novi Sad", Novi Sad, Serbia.
Food Environ Virol. 2015 Feb 17. doi: 10.1007/s12560-015-9187-3.
For the first time in Serbia, a small surveillance study was conducted in order to estimate the presence and frequency of occurrence of selected human [adenoviruses (HAdV), noroviruses, (NoV GI, NoV GII) and hepatitis A virus (HAV)], animal [porcine adenovirus (PAdV) and bovine polyomavirus (BPyV)] and zoonotic [hepatitis E virus (HEV)] viruses in selected surface waters. In total, 60 surface water samples were collected in two sampling occasions at 30 locations, with each sampling time being separated by 1-5 months. In addition, six sewage effluent samples were collected at one sampling site per each of the three tested town sewage systems, in two sampling occasions with 2 months intervals, before their discharge into the surface waters. The most prevalent virus found was HAdV which was detected in 43.33 % samples. NoV GII was found in 40 % samples. NoV GI was found in 10 % samples, and PAdV, BPyV and HEV were detected in 5 (8.33 %), 4 (6.67 %) and 2 (3.33 %) samples, respectively. HAV was not found in any of analysed surface waters or urban sewage samples. The obtained results confirm the presence of pathogenic enteric viruses of both human and animal origin in surface waters in Serbia indicating the existence of diverse contamination sources.
在塞尔维亚首次开展了一项小型监测研究，以评估选定的人类病毒（腺病毒、诺如病毒GI型、诺如病毒GII型和甲型肝炎病毒）、动物病毒（猪腺病毒和牛多瘤病毒）和人畜共患病毒（戊型肝炎病毒）在选定地表水的存在情况和发生频率。总共在30个地点分两次采集了60份地表水样本，每次采样时间间隔1 - 5个月。此外，在三个受试城镇污水系统的每个系统的一个采样点分两次采集了6份污水排放样本，采样间隔为2个月，这些污水排放样本在排入地表水之前进行采集。发现的最普遍的病毒是腺病毒，在43.33%的样本中检测到。诺如病毒GII型在40%的样本中被发现。诺如病毒GI型在10%的样本中被发现，猪腺病毒、牛多瘤病毒和戊型肝炎病毒分别在5份（8.33%）、4份（6.67%）和2份（3.33%）样本中被检测到。在所分析的任何地表水或城市污水样本中均未发现甲型肝炎病毒。所得结果证实塞尔维亚地表水中存在人类和动物源的致病性肠道病毒，表明存在多种污染源。
