Li B X, Ge J W, Li Y J
Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, 150030, Harbin, China.
Virology. 2007 Aug 15;365(1):166-72. doi: 10.1016/j.virol.2007.03.031. Epub 2007 Apr 30.
Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in piglets that leads to great economic losses in East Asia. It was reported that aminopeptidase N (APN) is the receptor for transmissible gastroenteritis virus (TGEV), human coronavirus 229E (HCoV-229E) and feline coronavirus (FeCoV) which all belong to group I coronavirus including as well as PEDV. It was also confirmed previously that porcine aminopeptidase N (pAPN) can bind to PEDV, and anti-pAPN antibodies may inhibit the combination. To investigate whether pAPN is a receptor for PEDV, we transfected MDCK cells with porcine aminopeptidase (pAPN) cDNA and this enabled non-susceptible cells to support PEDV replication and serial viral propagation. Moreover, the infection was blocked by antibodies against pAPN, implies the critical role of pAPN during virus entry. In addition, immunofluorescence assays for detection of pAPN and PEDV antigens, together with neutralization assays using antibodies against pAPN, further confirmed the correlation between pAPN expression and viral replication in pAPN-transfected MDCK cells. These results indicate that pAPN is a functional receptor for PEDV.
猪流行性腹泻病毒（PEDV）可导致仔猪致命性腹泻，在东亚地区造成巨大经济损失。据报道，氨肽酶N（APN）是传染性胃肠炎病毒（TGEV）、人冠状病毒229E（HCoV-229E）和猫冠状病毒（FeCoV）的受体，这些病毒均属于I群冠状病毒，PEDV也包含在内。先前也已证实，猪氨肽酶N（pAPN）可与PEDV结合，抗pAPN抗体可能会抑制这种结合。为了研究pAPN是否为PEDV的受体，我们用猪氨肽酶（pAPN）cDNA转染了MDCK细胞，这使得原本不敏感的细胞能够支持PEDV复制和病毒的连续传播。此外，针对pAPN的抗体可阻断感染，这表明pAPN在病毒进入过程中起关键作用。此外，用于检测pAPN和PEDV抗原的免疫荧光试验，以及使用抗pAPN抗体的中和试验，进一步证实了在pAPN转染的MDCK细胞中pAPN表达与病毒复制之间的相关性。这些结果表明，pAPN是PEDV的功能性受体。
